Quantitative detection of hepatitis B virus DNA in serum using chemiluminescence: comparison with radioactive solution hybridization assay
| Autor: | Marielle Cohard, Christine Baccard, Valerie Barlet, Jean-Pierre Zarski, Marie Ange Thelu, Jean Marie Seigneurin, Marie Jeanne Chaix |
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| Rok vydání: | 1994 |
| Předmět: |
Hepatitis B virus
Biology medicine.disease_cause Polymerase Chain Reaction law.invention Nucleic acid thermodynamics chemistry.chemical_compound law Virology medicine Humans Hepatitis B Antibodies Polymerase chain reaction Chemiluminescence Hepatitis B Surface Antigens Nucleic Acid Hybridization Reproducibility of Results Hepatitis B biology.organism_classification medicine.disease Hepatitis B Core Antigens Molecular biology Genetic Techniques Hepadnaviridae chemistry Carrier State DNA Viral Luminescent Measurements Solution hybridization DNA |
| Zdroj: | Journal of Virological Methods. 49:141-151 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(94)90038-8 |
| Popis: | A quantitative, non-radioactive hybrid capture HBV DNA assay (Digene Diagnostics), which uses an efficient solution hybridization procedure coupled to a sensitive chemiluminescent signal amplification system, was compared with the quantitative, radioactive solution hybridization assay (Genostics, Abbott Laboratories), in hepatitis B virus carriers, particularly in those undergoing antiviral therapy. The qualitative reproducibility of the chemiluminescent method, tested on 30 sera, was acceptable, with a reproducibility rate of 93.3%. A comparison of this hybrid capture HBV DNA assay with the radioactive test on 113 sera obtained from 48 patients (39 HBsAg-positive patients) gave a sensitivity of 87.2%, a specificity of 100% and an agreement between the two tests of 89.4% (101 sera including 82 HBV DNA positive and 19 negative samples). Changes in HBV DNA levels measured by the two assays showed a good correlation with each other during interferon therapy. However, the hybrid capture values were higher than the radioactive assay values, with the ratio of the two values being variable in the same patient during the course of treatment. The Genostics assay therefore seems to be a more accurate procedure for evaluating changes in viral replication, particularly at high HBV DNA levels. However, the hybrid capture method is faster and has the advantage of being a non-radioactive procedure. This chemiluminescent assay is easy to perform as a routine diagnostic procedure and may be a useful alternative to the radioactive solution hybridization method. |
| Databáze: | OpenAIRE |
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