Effects of isolation method and pre-treatment with ethylene glycol or raffinose before vitrification on in vitro viability of mouse preantral follicles
| Autor: | Yoshiyuki Takahashi, Seiji Katagiri, Masashi Nagano, Edwin C. Atabay, Mitsugu Hishinuma, Eufrocina P. Atabay |
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| Rok vydání: | 2007 |
| Předmět: |
Pre treatment
Male Ethylene Glycol Cell Survival Cell Separation General Biochemistry Genetics and Molecular Biology Andrology Tissue Culture Techniques chemistry.chemical_compound Mice Raffinose Ovarian Follicle Follicular phase medicine Animals Vitrification Dehydration General Medicine medicine.disease In vitro Mice Inbred C57BL chemistry Mice Inbred CBA Female Ethylene glycol Cytoplasmic Vacuolation |
| Zdroj: | Biomedical research (Tokyo, Japan). 28(3) |
| ISSN: | 0388-6107 |
| Popis: | Effects of isolation and vitrification protocols on follicular survival after warming were examined. Mouse preantral follicles enzymatically or mechanically isolated from ovaries of 12-day-old mice were exposed either to 2 M ethylene glycol (EG) for 2 or 5 min, or to ascending concentrations (0.15 then 0.3 M) of raffinose for 2 or 5 min each (2-2 and 5-5 min). They were then exposed to a vitrification solution (VS) composed of 6 M EG and 0.3 M raffinose for 0.5, 1, or 2 min before vitrification. Mechanically isolated follicles showed higher survival than enzymatically isolated follicles, regardless of periods of exposure to EG or raffinose and subsequent exposure to VS. After 10 days of culture, follicular growth and maturational ability of oocytes derived from vitrified follicles exposed to 2 M EG for 5 min and to VS for 1 min were higher than those from follicles exposed to raffinose solutions for 2-2 min and to VS for 1 min. Histological evaluation revealed that exposure of preantral follicles to raffinose solutions caused cytoplasmic vacuolation in granulosa cells which could be due to cellular shrinkage during dehydration; whereas, exposure to 2 M EG induced morphological alterations in follicles only to a lesser extent. |
| Databáze: | OpenAIRE |
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