Dietary Squalene Induces Cytochromes Cyp2b10 and Cyp2c55 Independently of Sex, Dose, and Diet in Several Mouse Models
| Autor: | Carmen Arnal, Araceli Sánchez-Ortiz, Clara Gabás-Rivera, María A. Navarro, Jesús Osada, Roberto Martínez-Beamonte, Joaquín C. Surra, Eduardo Romanos, María Jesús Rodríguez-Yoldi, Enrique Jurado-Ruiz, Cristina Andres-Lacueva |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Squalene medicine.medical_specialty Biology Transcriptome 03 medical and health sciences chemistry.chemical_compound Apolipoproteins E Lipid droplet Internal medicine Western diet medicine Animals Humans Castration Cytochrome P450 Family 2 Dietary fat 030109 nutrition & dietetics Apolipoprotein A-I Dose-Response Relationship Drug Hep G2 Cells Lipids Sexual hormones Mice Inbred C57BL Cytochrome P-450 CYP2B6 030104 developmental biology Endocrinology chemistry Gene Expression Regulation Liver Diet Western Dietary Supplements Steroid Hydroxylases Female Aryl Hydrocarbon Hydroxylases Food Science Biotechnology Olive oil Hormone |
| Zdroj: | Molecular nutritionfood research. 64(20) |
| ISSN: | 1613-4133 |
| Popis: | Scope To investigate the effects of squalene, the main hydrocarbon present in extra virgin olive oil, on liver transcriptome in different animal models and to test the influence of sex on this action and its relationship with hepatic lipids. Methods and results To this purpose, male C57BL/6J Apoe-deficient mice are fed a purified Western diet with or without squalene during 11 weeks and hepatic squalene content is assessed, so are hepatic lipids and lipid droplets. Hepatic transcriptomic changes are studied and confirmed by RT-qPCR. Dietary characteristics and influence of squalene doses are tested in Apoe-deficient on purified chow diets with or without squalene. These diets are also given to Apoa1 and wild-type mice on C57BL/6J background and to C57BL/6J xOla129 Apoe-deficient mice. Squalene supplementation increases its hepatic content without differences among sexes and hormonal status. The Cyp2b10 and Cyp2c55 gene expressions are significantly up-regulated by the squalene intake in all models, with independence of sex, sexual hormones, dietary fat content, genetic background and dose, and in Apoe-deficient mice consuming extra-virgin olive oil. Conclusion Hepatic squalene increases the expression of these cytochromes and their changes in virgin olive oil diets may be due to their squalene content. |
| Databáze: | OpenAIRE |
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