In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection
| Autor: | Mina Park, Gi Ru Shin, Hyeon Jin Ju, Hak Soo Choi, Ji Hoon Park, Myung Whan Suh, Moon Suk Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Depot
intratympanic injection 02 engineering and technology Near infrared fluorescence lcsh:Technology Article 03 medical and health sciences Tetrazine chemistry.chemical_compound 0302 clinical medicine depot Hyaluronic acid hyaluronic acid click-crosslinking General Materials Science 030223 otorhinolaryngology Nir fluorescence lcsh:Microscopy lcsh:QC120-168.85 lcsh:QH201-278.5 Chemistry lcsh:T 021001 nanoscience & nanotechnology lcsh:TA1-2040 near-infrared fluorescence Self-healing hydrogels lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Preclinical imaging Biomedical engineering |
| Zdroj: | Materials, Vol 13, Iss 3070, p 3070 (2020) Materials Volume 13 Issue 14 |
| ISSN: | 1996-1944 |
| Popis: | In this study, we developed injectable intratympanic hyaluronic acid (HA) depots for the treatment of hearing loss. We prepared an injectable click-crosslinking formulation by modifying HA with tetrazine (HA-TET) and trans-cyclooctene (HA-TCO), which crosslinked to form an HA depot (Cx-HA). Preparation of the click-crosslinking HA formulation was facile, and Cx-HA depot formation was reproducible. Additionally, the Cx-HA hydrogel was significantly stiffer than HA hydrogel. To monitor the degradation pattern of hydrogels, we mixed a zwitterionic near-infrared (NIR) fluorophore (e.g., ZW800-1C) in the click-crosslinking HA formulation. Then, HA-TET and HA-TCO solutions containing ZW800-1C were loaded separately into the compartments of a dual-barrel syringe for intratympanic injection. The Cx-HA depots formed quickly, and an extended residence time in the tympanic cavity was confirmed by performing NIR fluorescence imaging. We have successfully prepared an injectable click-crosslinking HA formulation that has promise as an intratympanic drug depot. |
| Databáze: | OpenAIRE |
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