Redox modulation of A-type K+ currents in pain-sensing dorsal root ganglion neurons
| Autor: | Chi-Pan Hsieh |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Small diameter
Redox modulation Pyridines Reducing agent Biophysics Pain Biochemistry Redox K currents Dithiothreitol Tosyl Compounds chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal Oxidizing agent medicine Animals Cysteine Neurons Afferent Rats Wistar Molecular Biology Chloramines Kv Channel-Interacting Proteins Cell Biology Anatomy Oxidants Rats medicine.anatomical_structure chemistry Kv1.4 Potassium Channel Oxidation-Reduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 370:445-449 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.03.097 |
| Popis: | Redox modulation of fast inactivation has been described in certain cloned A-type voltage-gated K(+) (Kv) channels in expressing systems, but the effects remain to be demonstrated in native neurons. In this study, we examined the effects of cysteine-specific redox agents on the A-type K(+) currents in acutely dissociated small diameter dorsal root ganglion (DRG) neurons from rats. The fast inactivation of most A-type currents was markedly removed or slowed by the oxidizing agents 2,2'-dithio-bis(5-nitropyridine) (DTBNP) and chloramine-T. Dithiothreitol, a reducing agent for the disulfide bond, restored the inactivation. These results demonstrated that native A-type K(+) channels, probably Kv1.4, could switch the roles between inactivating and non-inactivating K(+) channels via redox regulation in pain-sensing DRG neurons. The A-type channels may play a role in adjusting pain sensitivity in response to peripheral redox conditions. |
| Databáze: | OpenAIRE |
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