Evaluation of a new Argovit as an antiviral agent included in feed to protect the shrimp Litopenaeus vannamei against White Spot Syndrome Virus infection
| Autor: | Píndaro Álvarez-Ruiz, María Cristina Chávez-Sánchez, Nina Bogdanchikova, Carlos R. Romo-Quiñonez, Ana Ruth Álvarez-Sánchez, Claudio Humberto Mejía-Ruíz, Alexey Pestryakov |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
Feed additive animal structures White spot syndrome Litopenaeus lcsh:Medicine Marine Biology Biology General Biochemistry Genetics and Molecular Biology Virus AgNps 03 medical and health sciences Immune system Virology Bioassay Agricultural Science 030304 developmental biology 0303 health sciences General Neuroscience lcsh:R fungi 04 agricultural and veterinary sciences General Medicine biology.organism_classification Shrimp WSSV Toxicity 040102 fisheries Aquaculture Fisheries and Fish Science 0401 agriculture forestry and fisheries General Agricultural and Biological Sciences Biotechnology |
| Zdroj: | PeerJ PeerJ, Vol 8, p e8446 (2020) |
| ISSN: | 2167-8359 |
| Popis: | In this study, four experimental assays were conducted to evaluate the use of a new silver nanoparticle formulation named Argovit-4, which was prepared with slight modifications to enhance its biological activity against white spot syndrome virus (WSSV) in shrimp culture. The goals of these assays were to (1) determine the protective effect of Argovit-4 against WSSV, (2) determine whether Argovit-4 supplemented in feed exhibits toxicity towards shrimp, (3) determine whether Argovit-4 as antiviral additive in feed can prevent or delay/reduce WSSV-induced shrimp mortality, and (4) determine whether Argovit-4 supplemented in feed alters the early stages of the shrimp immune response. In bioassay 1, several viral inocula calibrated at 7 SID50(shrimp infectious doses 50% endpoint) were exposed to 40, 100, 200 and 1,000 ng/SID50of Ag+and then intramuscularly injected into shrimp for 96 h. In bioassay 2, shrimp were fed Argovit-4 supplemented in feed at different concentrations (10, 100 and 1,000 µg per gram of feed) for 192 h. In bioassay 3, shrimp were treated with Argovit-4 supplemented in feed at different concentrations and then challenged against WSSV for 192 h. In bioassay 4, quantitative real-time RT-qPCR was performed to measure the transcriptional responses of five immune-relevant genes in haemocytes of experimental shrimp treated with Argovit-4 supplemented in feed at 0, 6, 12, 24 and 48 h. The intramuscularly injected Argovit-4 showed a dose-dependent effect (p |
| Databáze: | OpenAIRE |
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