Perezone, from the gorgonian Pseudopterogorgia rigida, induces oxidative stress in human leukemia cells
| Autor: | Elthon G. Ferreira, Jose Delano Barreto Marinho-Filho, Letícia V. Costa-Lotufo, Alessandra L. Valverde, Carlos Margo R. Ribeiro, Ana Jérsia Araújo, Paula C. Jimenez, Diego Veras Wilke, Paula Abreu, Leandro S. Pinheiro, Rosangela de Almeida Epifanio, Juliana W. Amorim |
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| Jazyk: | angličtina |
| Předmět: |
food.ingredient
Cytotoxicity lcsh:RS1-441 Biology Pseudopterogorgia medicine.disease_cause lcsh:Pharmacy and materia medica Pharmacology Toxicology and Pharmaceutics(all) food Botany medicine Cytotoxic T cell MTT assay Leucemia General Pharmacology Toxicology and Pharmaceutics Leukemia ESTRESSE OXIDATIVO ROS Free radical scavenger Molecular biology Gorgonian coral Oxidative Stress Cell culture Marine natural products Quinone Intracellular Oxidative stress |
| Zdroj: | Revista Brasileira de Farmacognosia, Vol 25, Iss 6, Pp 634-640 Revista Brasileira de Farmacognosia, Vol 25, Iss 6, Pp 634-640 (2015) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Revista Brasileira de Farmacognosia, Volume: 25, Issue: 6, Pages: 634-640, Published: DEC 2015 Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC Revista Brasileira de Farmacognosia v.25 n.6 2015 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
| Popis: | Four bisabolanes 1–4, including perezone (1) and triacetyl perezone (2), were isolated through a bioassay-guided fractionation of the extract obtained from the Caribbean gorgonian coral Pseudopterogorgia rigida collected during an expedition cruise to the Bahamas. All isolated compounds showed to be cytotoxic toward panel of four human tumor cell lines, as quantified by the MTT assay after 72 h incubation. Perezone (1), the most active one, was further analyzed, showing to be cytotoxic, but not selective, in a 12-cell line panel comprising tumor and non-tumor, as well as human and murine cells. Additionally, 1 was assayed for cytotoxicity against HL-60 leukemic cells. Pre-treatment with an acute free radical scavenger (l-NAC) before exposure of cells to perezone virtually eliminated the generation of intracellular ROS and lessened its severe cytotoxicity. The protective effect delivered by l-NAC evidences that the mechanism of perezone-induced cytotoxicity is partially associated to production of ROS and a consequent induction of oxidative stress. Keywords: Gorgonian coral, Marine natural products, Cytotoxicity, Quinone, ROS |
| Databáze: | OpenAIRE |
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