Identification and Elimination of the Clinically Relevant Multi-Resistant Environmental Bacteria Ralstonia insidiosa in Primary Cell Culture
| Autor: | Melinda Ahmels, Dennis Nurjadi, Sébastien Boutin, Katja Schmidt, Daniel Hasche |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.drug_class 030106 microbiology Antibiotics Ralstonia insidiosa Microbiology Article 03 medical and health sciences bacterial contamination multidrug resistance Virology medicine lcsh:QH301-705.5 Whole genome sequencing biology fungi Biofilm food and beverages biology.organism_classification Multiple drug resistance Ciprofloxacin 030104 developmental biology lcsh:Biology (General) Cell culture Bacteria medicine.drug primary cell culture |
| Zdroj: | Microorganisms Volume 8 Issue 10 Microorganisms, Vol 8, Iss 1599, p 1599 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8101599 |
| Popis: | In times of spreading multidrug-resistant bacteria, species identification and decontamination of cell cultures can be challenging. Here, we describe a mobile cell culture contaminant with &ldquo black dot&rdquo like microscopic appearance in newly established irreplaceable hybridoma cell lines and its identification. Using 16S rRNA gene sequencing, species-specific PCRs, whole genome sequencing (WGS), and MALDI-TOF mass spectrometry, the contaminant was identified as the ubiquitous environmental and clinically relevant Gram-negative bacterium Ralstonia insidiosa (R. insidiosa), a strong biofilm producer. Further characterizations by transmission electron microscopy (TEM) and biochemical API test were not conclusive. Whole genome sequencing of our R. insidiosa isolate revealed numerous drug-resistance determinants. Genome-wide comparison to other Ralstonia species could not unambiguously designate our isolate to R. insidiosa (< 95% average nucleotide identity) suggesting a potential novel species or subspecies, closely related to R. insidiosa and R. pickettii. After determining the antibiotic susceptibility profile, the hybridoma cell culture was successfully decontaminated with ciprofloxacin without affecting antibody production. |
| Databáze: | OpenAIRE |
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