Paracrine Activity from Adipose-Derived Stem Cells on In Vitro Wound Healing in Human Tympanic Membrane Keratinocytes
| Autor: | Peder Aabel, Sharon L. Redmond, Huan Ting Ong, Rodney J. Dilley, Marcus D. Atlas, Robert J. Marano, Magnus von Unge |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Keratinocytes Tympanic Membrane medicine.medical_treatment Paracrine Communication Biology 03 medical and health sciences Paracrine signalling 0302 clinical medicine Medisinske Fag: 700 [VDP] Cell Movement medicine Humans VDP::Medisinske Fag: 700 Artikkel Cell Proliferation Wound Healing Gene Expression Profiling Stem Cells Mesenchymal stem cell Cell Biology Hematology Cell Hypoxia Cell biology HaCaT 030104 developmental biology Cytokine medicine.anatomical_structure Gene Ontology Adipose Tissue 030220 oncology & carcinogenesis Culture Media Conditioned Immunology Cytokines Intercellular Signaling Peptides and Proteins Stem cell Wound healing Keratinocyte Developmental Biology |
| Zdroj: | Stem cells and development. 26(6) |
| ISSN: | 1557-8534 |
| Popis: | Stem cell therapies for tympanic membrane repair have shown initial experimental success using mesenchymal stem cells in rat models to promote healing; however, the mechanisms providing this benefit are not known. We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSCs) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalized human keratinocytes (HaCaT). ADSC conditioned media (CMADSC) were assessed for paracrine activity on keratinocyte proliferation and migration, with hypoxic conditions for ADSC culture used to generate contrasting effects on cytokine gene expression. Keratinocytes cultured in CMADSC showed a significant increase in cell number compared to serum-free cultures and further significant increases in hypoxic CMADSC. Assessment of ADSC gene expression on a cytokine array showed a range of wound healing cytokines expressed and under stringent hypoxic and serum-free conditions was upregulated (VEGF A, MMP9, Tissue Factor, PAI-1) or downregulated (CXCL5, CCL7, TNF-α). Several of these may contribute to the activity of conditioned media on the keratinocytes with potential applications in TM perforation repair. VEGFA protein was confirmed by immunoassay to be increased in conditioned media. Together with gene regulation associated with hypoxia in ADSCs, this study has provided several strong leads for a stem cell–derived approach to TM wound healing. |
| Databáze: | OpenAIRE |
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