Evaluation of five temperature-sensitive mutants of respiratory syncytial virus in primates: II. Genetic analysis of virus recovered during infection
| Autor: | Robert M. Chanock, D. Lewis Sly, D. A. Prevar, Linda S. Richardson, William T. London, R. B. Belshe, Ena Camargo |
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| Rok vydání: | 1978 |
| Předmět: |
Pan troglodytes
Mutant Virulence medicine.disease_cause Respirovirus Infections Genetic analysis Virus In vivo Nasopharynx Virology biology.animal medicine Animals Primate Respiratory Tract Infections Mutation biology Temperature Haplorhini biology.organism_classification Respiratory Syncytial Viruses Infectious Diseases |
| Zdroj: | Journal of Medical Virology. 3:101-110 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.1890030203 |
| Popis: | Five temperature-sensitive (ts) mutants of respiratory syncytial (RS) virus (ts-1, ts-1 NG-1, ts-1 NG-16, ts-2, and ts-7), previously evaluated forinfectivity and virulence in chimpanzees and owl monkeys, were also assayed for in vivo genetic stability. None of the five mutants tested was completely stable genetically. Thus, virus which had lost some or all of the ts property was recovered from each infected chimpanzee. Significantly, each ts-1 NG-1 isolate retained some degree of temperature sensitivity and hence was not true wild-type virus. Clonal analysis of viruses shed by ts-1, ts-1 NG-1, ts-1 NG-16, or ts-7 infected chimpanzees indicated that in most instances only a minority of the virus shed was altered genetically. Of five chimpanzees infected with the ts-2 mutant, three shed only ts virus, and the remaining two chimpanzees shed only ts+ virus. Such ts+ virus proved to be avirulent when evaluated in chimpanzees or owl monkeys, indicating that loss of the ts property did not restore virulence. Based upon these findings, the ts-2 mutant appears to be a suitable candidate for clinical trials in man. |
| Databáze: | OpenAIRE |
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