Dentin bonding: effect of tubule orientation on hybrid-layer formation

Autor: Ivo Krejci, Felix Lutz, Peter Schupbach
Rok vydání: 1997
Předmět:
Dental Pulp/physiology
Materials science
Collagen/ultrastructure
Surface Properties
Hydrostatic pressure
Smear layer
Dental bonding
Resin Cements/chemistry
Dentin-Bonding Agents/chemistry
Acid Etching
Dental
stomatognathic system
Adhesives
Microscopy
Adhesives/chemistry
Hydrostatic Pressure
Dentin
medicine
Humans
Composite material
General Dentistry
Dental Pulp
Fluorescent Dyes
Maleates/chemistry
Microscopy
Confocal
Rhodamines
Maleates
Dental Bonding
Anatomy
ddc:617.6
Resin Cements
Microscopy
Electron
stomatognathic diseases
medicine.anatomical_structure
Dentinal Tubule
Tubule
Microscopy
Fluorescence
Transmission electron microscopy
Dentin-Bonding Agents
Smear Layer
Methacrylates
Fluorescein
Methacrylates/chemistry
Collagen
Dental Cavity Preparation
Porosity
Dentin/physiology/ultrastructure
Zdroj: European Journal of Oral Sciences, Vol. 105, No 4 (1997) pp. 344-352
ISSN: 1600-0722
0909-8836
DOI: 10.1111/j.1600-0722.1997.tb00251.x
Popis: In an attempt to compare the morphology of the resin-dentin interface in areas where the dentinal tubules run perpendicularly or at an angle to the cavity surface with that of areas where they run parallel to it, we studied a dentin adhesive system using transmission electron microscopy and fluorescence confocal laser scanning microscopy. The design of the study included the simulation of the normal hydrostatic pressure within the pulp and the dentinal tubules. Following acid etching of the dentinal surface with maleic acid/HEMA, the smear layer was removed, and a superficial zone was demineralized in such a way that the exposed collagenous dentin matrix retained its integrity. Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred not only vertically via surface porosities, but mainly laterally, via the dentinal tubules. The adhesive resin labeled with fluorescein completely infiltrated the demineralized layer, thereby forming a hybrid layer. The orientation of the dentinal tubules had a profound effect on the formation of the hybrid layer. In areas with perpendicular tubule orientation, the layer was 3.2 +/- 0.8 microns thick, showing solid 27.2 +/- 0.8 microns long resin tags in the dentinal tubules, and a network of tiny tags in their side-branches. In areas with parallel tubule orientation the layer was significantly thinner (1.3 +/- 0.6 microns) and resin tags were absent.
Databáze: OpenAIRE
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