Immunoglobulin G immobilization on PVDF surface
| Autor: | Mihaela Baican, Manuela T. Nistor, Cornelia Vasile, Elena Pâslaru, Elena Gabriela Hitruc, Fabienne Poncin-Epaillard |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Surface Properties
Microscopy Atomic Force Immunoglobulin G Colloid and Surface Chemistry X-ray photoelectron spectroscopy Spectroscopy Fourier Transform Infrared Polymer chemistry Humans Antigens Least-Squares Analysis Physical and Theoretical Chemistry Staphylococcal Protein A chemistry.chemical_classification Principal Component Analysis Spectroscopy Near-Infrared biology Chemistry Photoelectron Spectroscopy Discriminant Analysis Substrate (chemistry) Surfaces and Interfaces General Medicine Quartz crystal microbalance Polymer Immobilized Proteins Membrane Chemical engineering Quartz Crystal Microbalance Techniques biology.protein Surface modification Polyvinyls Protein A Protein Binding Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 115:139-149 |
| ISSN: | 0927-7765 |
| Popis: | Immobilization of antibody molecules onto hydrophobic polymeric surfaces with disordered orientation is something unwanted in many applications. To overcome this drawback, controlled immunoglobulin G (IgG) immobilization onto poly(vinylidene fluoride) surface was investigated in this paper. A two-step process involving radiofrequency plasma pretreatment for polymer surface functionalization, followed by coupling reaction was developed, after which immunoglobulin G was immobilized onto the surface directly or via protein-A. IR and XPS data proved that the process is more efficient when the radiofrequency plasma pretreatment was performed using N 2 and N 2 /H 2 as discharge gases. NIR-CI, AFM and XPS surface evaluation revealed that immobilization of IgG onto N 2 /H 2 plasma-treated PVDF via grafted protein-A was achieved with an ends-on orientation, leaving available the antigen binding sites of IgG. This procedure could be a promising route for the preparation of oriented IgG assembly onto PVDF, useful in biomedical, membranes or sensors applications. QCM results showed a better antibody–antigen interaction when IgG immobilization onto PVDF substrate is mediated by protein A. |
| Databáze: | OpenAIRE |
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