A novel reversed-phase HPLC method for the determination of urinary creatinine by pre-column derivatization with ethyl chloroformate: comparative studies with the standard Jaffé and isotope-dilution mass spectrometric assays
| Autor: | Elvis M. K. Leung, Wan Chan |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Creatinine Chromatography Formic Acid Esters Renal function Indicator Dilution Techniques Urine Reversed-phase chromatography Isotope dilution Biochemistry High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Rats Rats Sprague-Dawley chemistry.chemical_compound chemistry Limit of Detection Animals Ethyl chloroformate Derivatization Chromatography High Pressure Liquid |
| Zdroj: | Analytical and bioanalytical chemistry. 406(6) |
| ISSN: | 1618-2650 |
| Popis: | Creatinine is an important biomarker for renal function diagnosis and normalizing variations in urinary drug/metabolites concentration. Quantification of creatinine in biological fluids such as urine and plasma is important for clinical diagnosis as well as in biomonitoring programs and urinary metabolomics/metabonomics research. Current methods for creatinine determination either are nonselective or involve the use of expensive mass spectrometers. In this paper, a novel reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of creatinine of high hydrophilicity by pre-column derivatization with ethyl chloroformate is presented. N-Ethyloxycarbonylation of creatinine significantly enhanced the hydrophobicity of creatinine, facilitating its chromatographic retention as well as quantification by HPLC. Factors governing the derivatization reaction were studied and optimized. The developed method was validated and applied for the determination of creatinine in rat urine samples. Comparative studies with isotope-dilution mass spectrometric method revealed that the two methods do not yield systematic differences in creatinine concentrations, indicating the HPLC method is suitable for the determination of creatinine in urine samples. |
| Databáze: | OpenAIRE |
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