Blood-pressure lowering efficacy of winged bean seed hydrolysate in spontaneously hypertensive rats, peptide characterization and a toxicity study in Sprague-Dawley rats
| Autor: | Nor Fazila Sulaiman, Mohamad Ariff Hanafi, Shyan Yea Chay, Annas Salleh, Najib Zainal Abidin, Nazamid Saari, Mohammad Zarei |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Protein Hydrolysates Peptide Angiotensin-Converting Enzyme Inhibitors Blood Pressure 010501 environmental sciences Pharmacology 01 natural sciences Peptide Mapping Hydrolysate Rats Sprague-Dawley 03 medical and health sciences Functional food In vivo Rats Inbred SHR Renin Animals Humans Antihypertensive Agents 0105 earth and related environmental sciences chemistry.chemical_classification Plant Extracts Fabaceae General Medicine Prodrug In vitro Rats Molecular Docking Simulation 030104 developmental biology Blood pressure chemistry Toxicity Hypertension Seeds Female Peptides Food Science |
| Zdroj: | Foodfunction. 9(3) |
| ISSN: | 2042-650X |
| Popis: | Winged bean seed (WBS) is an underutilized tropical crop. The current study evaluates its potential to reduce blood pressure (BP) in spontaneously hypertensive rats and finds that it reduces BP significantly, in a dose-dependent manner. Five peptides with the sequences, RGVFPCLK, TQLDLPTQ, EPALVP, MRSVVT and DMKP, have been characterized in terms of their stability against ACE via in vitro and in silico modelling. All peptides exhibited IC50 values between 0.019 and 6.885 mM and various inhibitory modes, including substrate, prodrug and true inhibitor modes. The toxicity status of non-Current Good Manufacturing Practice (non-CGMP) peptides is evaluated and the results show that such peptides are toxic, and thus are not suitable to be tested in animals, particularly in repeated-dose studies. In short, WBS hydrolysate demonstrated in vitro ACE inhibitory properties and in vivo blood pressure lowering efficacy in rat models, fostering its potential as a functional food ingredient. Non-CGMP grade peptides are toxic and unfit for testing in animal models. |
| Databáze: | OpenAIRE |
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