Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns
| Autor: | Ana C. Calpena, José L Soriano, Beatriz Clares, Òscar Domènech, María Luisa Garduño-Ramírez, María J. Rodríguez-Lagunas, Nuria Bozal-de Febrer |
|---|---|
| Přispěvatelé: | [Soriano,JL, Clares,B] Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain. [Calpena,AC, Domènech,Ò] Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain. [Calpena,AC, Domènech,Ò, Clares,B] Nanoscience & Nanotechnology Institute (IN2UB), University of Barcelona, Barcelona, Spain. [Rodríguez-Lagunas,MJ] Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain. [Rodríguez-Lagunas,MJ] Nutrition and Food Safety Research Institute (INSA-UB), Santa Coloma de Gramenet, Spain. [Bozal-de Febrer,N] Department of Biology, Healthcare and the Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain. [Garduño-Ramírez,ML] Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico. [Clares,B] Biosanitary Institute of Granada (ibs.GRANADA), Granada, Spain. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Vitamines
antioxidant Antioxidant medicine.medical_treatment Microscopía de fuerza atómica lcsh:RS1-441 Pharmaceutical Science melatonin Endogeny Antioxidantes 02 engineering and technology Pharmacology Vitaminas Antioxidants Melatonina Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants [Medical Subject Headings] Organisms::Eukaryota::Animals [Medical Subject Headings] Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Growth Substances::Micronutrients::Vitamins [Medical Subject Headings] Melatonin Skin Skin repair Microscopy 0303 health sciences Cicatrización de heridas integumentary system Chemistry Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy Electron::Microscopy Electron Scanning [Medical Subject Headings] Vitamins vitamins healing 021001 nanoscience & nanotechnology Antimicrobial Microscòpia Phenomena and Processes::Biological Phenomena::Biological Processes::Regeneration::Wound Healing [Medical Subject Headings] Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents [Medical Subject Headings] 0210 nano-technology medicine.drug skin Healing Silver sulfadiazine Article lcsh:Pharmacy and materia medica 03 medical and health sciences ATF microscopy Psychiatry and Psychology::Behavior and Behavior Mechanisms::Motivation::Goals [Medical Subject Headings] In vivo medicine Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques Analytical::Spectrum Analysis [Medical Subject Headings] 030304 developmental biology Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats [Medical Subject Headings] Chemicals and Drugs::Organic Chemicals::Amides::Sulfonamides::Sulfanilamides::Sulfadiazine::Silver Sulfadiazine [Medical Subject Headings] Wound healing Chemicals and Drugs::Hormones Hormone Substitutes and Hormone Antagonists::Hormones::Melatonin [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy::Microscopy Scanning Probe::Microscopy Atomic Force [Medical Subject Headings] Piel |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Pharmaceutics Pharmaceutics, Vol 13, Iss 8, p 8 (2021) Dipòsit Digital de la UB Universidad de Barcelona Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Volume 13 Issue 1 |
| DOI: | 10.3390/pharmaceutics13010008 |
| Popis: | The main goal of this work is the study of the skin wound healing efficacy of an antioxidant cocktail consisting of vitamins A, D, E and the endogenous pineal hormone melatonin (MLT), with all of these loaded into a thermosensitive hydrogel delivery system. The resulting formulation was characterized by scanning electron microscopy. The antioxidant efficacy and microbiological activity against Gram positive and Gram negative strains were also assayed. The skin healing efficacy was tested using an in vivo model which included histological evaluation. Furthermore, atomic force microscopy was employed to evaluate the wound healing efficacy of rat skin burns through the determination of its elasticity at the nanoscale using force spectroscopy analysis. The resulting hydrogel exhibited sol state at low temperature and turned into a gel at 30 ± 0.2 ° C. The hydrogel containing the antioxidant cocktail showed higher scavenging activity than the hydrogel containing vitamins or MLT, separately. The formulation showed optimal antimicrobial activity. It was comparable to a commercial reference. It was also evidenced that the hydrogel containing the antioxidant cocktail exhibited the strongest healing process in the skin burns of rats, similar to the assayed commercial reference containing silver sulfadiazine. Histological studies confirmed the observed results. Finally, atomic force microscopy demonstrated a similar distribution of Young&rsquo s modulus values between burned skin treated with the commercial reference and burned skin treated with hydrogel containing the antioxidant cocktail, and all these with healthy skin. The use of an antioxidant cocktail of vitamins and MLT might be a promising treatment for skin wounds for future clinical studies. |
| Databáze: | OpenAIRE |
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