Chronic obstructive pulmonary disease upper airway microbiome is associated with select clinical characteristics

Autor:	Richard E. Isaacson, Alexa A. Pragman, Katherine A. Knutson, Shane W. Hodgson, Trevor J. Gould, Cavan S. Reilly, Chris H. Wendt
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Male 0301 basic medicine Pulmonology Physiology Prevotella Pathology and Laboratory Medicine Lung and Intrathoracic Tumors Pulmonary Disease Chronic Obstructive 0302 clinical medicine Medicine and Health Sciences Medicine COPD Multidisciplinary Ecology Microbiota Smoking Genomics Middle Aged Body Fluids Bacterial Pathogens 3. Good health Shannon Index Oncology Medical Microbiology Laryngeal Mucosa Female Anatomy Pathogens Simpson Index medicine.symptom Research Article Lung microbiome medicine.medical_specialty Ecological Metrics Chronic Obstructive Pulmonary Disease Science Microbial Genomics Disease cluster Microbiology Lung Disorder 03 medical and health sciences Internal medicine Genetics Humans Microbiome Microbial Pathogens Aged Bacteria business.industry Ecology and Environmental Sciences Sputum Organisms Biology and Life Sciences Streptococcus Cancers and Neoplasms Species Diversity medicine.disease respiratory tract diseases Mucus Nasal Mucosa 030104 developmental biology 030228 respiratory system Metagenome Airway business Dysbiosis
Zdroj:	PLoS ONE, Vol 14, Iss 7, p e0219962 (2019) PLoS ONE
ISSN:	1932-6203
Popis:	BackgroundChronic obstructive pulmonary disease (COPD) is an inflammatory lung disorder associated with lung microbiome dysbiosis. Although the upper airway microbiome is the source of the lung microbiome, the relationships between the oral, nasal, and sputum microbiota are incompletely understood. Our objective was to determine features that differentiate the oral, nasal, and sputum microbiome among subjects with stable COPD.MethodsWe recruited 15 current or former smokers to provide oral and sputum samples on day 1. On day 2, another oral sample and a nasal sample were obtained. Each sample and control underwent DNA extraction, 16S V4 rRNA amplification, 16S V4 sequencing, and qPCR of 16S rRNA. Data were analyzed using dada2 and R.ResultsMost (14 of 15) subjects were male with a mean age of 65.2. One subject had no pulmonary obstruction, while 5 had mild COPD, 7 had moderate COPD, and 2 had severe COPD. Three subjects (20%) were current tobacco users and 2 subjects (13%) used inhaled corticosteroids (ICS). Subjects had a mean of 49.1 pack-years of tobacco exposure. Bacterial biomass was associated with anatomic site, but no differences in biomass were observed with age, FEV1 percent predicted (FEV1pp), ICS use, smoking status, or edentulous state. Shannon index was associated with site (lower nasal diversity than oral and sputum diversity, pConclusionsAmong the upper airway microbiota of COPD subjects, anatomic site was associated with bacterial biomass, Shannon diversity, and β-diversity. ICS use and edentulous state were both associated with β-diversity. Age was associated with taxa relative abundance in oral and sputum samples, while FEV1pp was associated with taxa relative abundance in sputum samples only.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30afba2a7e77e0eece3fba1b319efa85 https://doaj.org/article/8d4ffefc53a64e1996c162930124d0de Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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