Cell/surface interactions on laser micro-textured titanium-coated silicon surfaces
| Autor: | Jianbo Chen, Mingwei Li, Aboubaker Beye, Wole Soboyejo, Steven Mwenifumbo |
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| Rok vydání: | 2007 |
| Předmět: |
Silicon
Materials science Phalloidine Surface Properties Cell Culture Techniques Biomedical Engineering Biophysics chemistry.chemical_element Bioengineering Nanotechnology Models Biological Biomaterials Focal adhesion Imaging Three-Dimensional Coated Materials Biocompatible Cell Movement Cell Line Tumor Microscopy Cell Adhesion Image Processing Computer-Assisted Humans Cytoskeleton Cell adhesion Cell Size Fluorescent Dyes Titanium Osteosarcoma Osteoblasts biology Rhodamines Lasers Adhesion Vinculin Actins Microscopy Fluorescence chemistry Fluorescent Antibody Technique Direct biology.protein |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 18:9-23 |
| ISSN: | 1573-4838 0957-4530 |
| DOI: | 10.1007/s10856-006-0658-9 |
| Popis: | This paper examines the effects of nano-scale titanium coatings, and micro-groove/micro-grid patterns on cell/surface interactions on silicon surfaces. The nature of the cellular attachment and adhesion to the coated/uncoated micro-textured surfaces was elucidated by the visualization of the cells and relevant cytoskeletal & focal adhesion proteins through scanning electron microscopy and immunofluorescence staining. Increased cell spreading and proliferation rates are observed on surfaces with 50 nm thick Ti coatings. The micro-groove geometries have been shown to promote contact guidance, which leads to reduced scar tissue formation. In contrast, smooth surfaces result in random cell orientations and the increased possibility of scar tissue formation. Immunofluorescence cell staining experiments also reveal that the actin stress fibers are aligned along the groove dimensions, with discrete focal adhesions occurring along the ridges, within the grooves and at the ends of the cell extensions. The implications of the observed cell/surface interactions are discussed for possible applications of silicon in implantable biomedical systems. |
| Databáze: | OpenAIRE |
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