Neuropathological and genomic characterization of glioblastoma‐induced rat model: How similar is it to humans for targeted therapy?
| Autor: | Saeid Ghavami, Hassan Nikuinejad, Sahar Kiani, Amir Ali Hamidieh, Farzaneh Sharifzad, Mehdi Noureddini, Esmaeil Fakharian, Marzieh Ebrahimi, Marek J. Łos, Javad Verdi, Adeleh Taghikhani, Saeid Mardpour, Soura Mardpour, Somayeh Vafaei, Hamed Yasavoli‐Sharahi, Reza khellat, Yasaman Heydari |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology medicine.medical_treatment Clinical Biochemistry Rat model Central nervous system Down-Regulation Genomics Computational biology Biology Targeted therapy 03 medical and health sciences 0302 clinical medicine Species Specificity Cell Line Tumor medicine Animals Humans Protein Interaction Maps Pathological Drug discovery Computational Biology Neoplasms Experimental Cell Biology medicine.disease Rats Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Tumor progression 030220 oncology & carcinogenesis Glioblastoma Transcriptome |
| Zdroj: | Journal of Cellular Physiology. 234:22493-22504 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.28813 |
| Popis: | Glioblastoma multiforme (GBM) is a unique aggressive tumor and mostly develops in the brain, while rarely spreading out of the central nervous system. It is associated with a high mortality rate; despite tremendous efforts having been made for effective therapy, tumor recurrence occurs with high prevalence. To elucidate the mechanisms that lead to new drug discovery, animal models of tumor progression is one of the oldest and most beneficial approaches to not only investigating the aggressive nature of the tumor, but also improving preclinical research. It is also a useful tool for predicting novel therapies' effectiveness as well as side effects. However, there are concerns that must be considered, such as the heterogeneity of tumor, biological properties, pharma dynamic, and anatomic shapes of the models, which have to be similar to humans as much as possible. Although several methods and various species have been used for this approach, the real recapitulation of the human tumor has been left under discussion. The GBM model, which has been verified in this study, has been established by using the Rat C6 cell line. By exploiting bioinformatic tools, the similarities between aberrant gene expression and pathways have been predicted. In this regard, 610 common genes and a number of pathways have been detected. Moreover, while magnetic resonance imaging analysis enables us to compare tumor features between these two specious, pathological findings provides most of the human GBM characteristics. Therefore, the present study provides genomics, pathologic, and imaging evidence for showing the similarities between human and rat GBM models. |
| Databáze: | OpenAIRE |
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