Isolation, purification and characterization of β-hCGRP from human spinal cord
| Autor: | Iain MacIntyre, Howard R. Morris, Sunil J. Wimalawansa, Anthony Etienne, Maria Panico, I Blench |
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| Rok vydání: | 1990 |
| Předmět: |
Calcitonin Gene-Related Peptide
Molecular Sequence Data Radioimmunoassay Biophysics Sequence (biology) Peptide Mass spectrometry Biochemistry Gas Chromatography-Mass Spectrometry Radioligand Assay medicine Humans Nucleotide Amino Acid Sequence Cyanogen Bromide Disulfides Molecular Biology Peptide sequence chemistry.chemical_classification Chromatography Chemistry Cell Biology Fast atom bombardment Spinal cord Peptide Fragments Amino acid Freeze Drying medicine.anatomical_structure Spinal Cord Chromatography Gel |
| Zdroj: | Biochemical and Biophysical Research Communications. 167:993-1000 |
| ISSN: | 0006-291X |
| Popis: | Human β-calcitonin gene-related peptide (β-hCGRP) was isolated and purified from spinal cord. The complete characterization of this material was based on definitive mass analysis by fast atom bombardment mass spectrometry together with gas phase sequencing. Combining these data we have characterized the structure of β-hCGRP including the C-terminal sequence, the presence of the S-S bridge and of phenylalanineamide as the C-terminal amino acid, and fully confirmed the amino acid sequence predicted from the nucleotide analysis. |
| Databáze: | OpenAIRE |
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