Inhibition of Toll Like Signaling Pathway Is Associated With Genomic Instability in Rat Liver Exposed to Crack Cocaine
| Autor: | Milena de Barros Viana, Ingra Tais Malacarne, Regina Cláudia Barbosa da Silva, Laís Vales Mennitti, Daniel Vitor de Souza, Daniel Araki Ribeiro, Luciana Pellegrini Pisani, Dos Anjos Barbara Rosario |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
medicine.medical_specialty Karyolysis media_common.quotation_subject Binucleated cells Genomic Instability General Biochemistry Genetics and Molecular Biology Internal medicine medicine Animals Crack cocaine media_common Cell Nucleus Pharmacology Chemistry Abstinence Rats Endocrinology Liver Rat liver Crack Cocaine Signal transduction Micronucleus Pyknosis Signal Transduction Research Article |
| Zdroj: | In Vivo |
| ISSN: | 1791-7549 0258-851X |
| DOI: | 10.21873/invivo.12546 |
| Popis: | Background/aim The aim of the present study was to investigate the biological effects of subacute crack cocaine exposure in rat liver. Material and methods A total of 32 rats were distributed into four groups (n=8): Experimental group 1 (G1) and Experimental group 2 (G2): rats received 18 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day, group G2 remained 72 h without exposure after the experimental period (5 days)(abstinence); Experimental group 3 (G3): rats received 36 mg/kg of body weight (b.w) of crack cocaine for 5 days, once a day; Control Group (CTRL): rats received only the vehicle (DMSO) administered by the intraperitoneal (i.p) route for 5 days, once a day. Results All groups exposed to crack cocaine had an increase in the number of micronucleated hepatocytes and binucleated cells only in the highest tested dose (36 mg/kg). Karyolysis had an increase in the 18 mg/kg dose, in the abstinence group (G2), and 36 mg/kg group (G3); whereas pyknotic nuclei had an increase in the G2 group. The group exposed to 18 mg/kg of crack cocaine also showed high 8 OHdG expression. The p-NF-κB p65 protein decreased in the groups exposed to crack cocaine at doses of 18 and 36 mg/kg, as well as in the abstinence group. MyD88 was also found decreased in the group exposed to crack cocaine at 18 mg/kg. Conclusion Crack cocaine inhibited toll like signaling pathway whilst being associated with genomic instability in rat liver cells. |
| Databáze: | OpenAIRE |
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