A novel site-specific chemical conjugation of IgG antibodies by affinity peptide for immunoassays
| Autor: | Naoto Ishikawa, Abdur Rafique, Yuji Ito, Satoka Mori, Arisa Abe |
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| Rok vydání: | 2020 |
| Předmět: |
animal structures
Immunoconjugates Peptide Enzyme-Linked Immunosorbent Assay 010402 general chemistry 01 natural sciences Biochemistry Immunoglobulin G Mice Staphylococcus aureus protein A medicine Animals Humans Molecular Biology chemistry.chemical_classification Immunoassay biology medicine.diagnostic_test 010405 organic chemistry fungi Antibodies Monoclonal Chemical conjugation General Medicine 0104 chemical sciences Immobilized Proteins chemistry biology.protein Electrophoresis Polyacrylamide Gel Antibody Protein A Peptides Latex Fixation Tests Conjugate Protein Binding |
| Zdroj: | Journal of biochemistry. 169(1) |
| ISSN: | 1756-2651 |
| Popis: | Recently, there has been an increasing interest in site-specific modifications of antibodies used in immunoassays for disease diagnosis and as antibody therapeutics, such as antibody−drug conjugates. Previously, we established a site-specific chemical conjugation system using an IgG-Fc binding chemical conjugation affinity peptide (CCAP). CCAP could be used only for the modification of human IgG owing to the lack of affinity of CCAP to rodent IgG molecules. In this study, novel CCAP reagents are proposed, which can be used for both human and mouse IgG, based on the Staphylococcus aureus protein A domain-derived affinity peptides Z34C and Z33. Compared with the activity of a conventional randomly modified antibody, mouse IgG modified using this method had favourable features in two immunoassays, demonstrating the advantages of the proposed CCAP method in preserving antibody functionality during conjugation. |
| Databáze: | OpenAIRE |
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