Calcium Dependent CAMTA1 in Adult Stem Cell Commitment to a Myocardial Lineage

Autor: Page A.W. Anderson, Nenad Bursac, Jian Ping Jin, Raymond G. Fox, Gwyn L. Esch, Nobuyuo Maeda, Rob Aldina, Mary R. Hutson, Barbara J. Muller-Borer, Woohyun Woon, Neal Shepherd, Nadia N. Malouf, Margaret L. Kirby, Sylvia Hiller
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Microarrays
Cellular differentiation
lcsh:Medicine
Gene Expression
Stem cell factor
Cell Communication
Cardiovascular
Mice
0302 clinical medicine
Molecular Cell Biology
Signaling in Cellular Processes
Myocytes
Cardiac
lcsh:Science
0303 health sciences
Induced stem cells
Multidisciplinary
Stem Cells
Cell Differentiation
Neural stem cell
Signaling Cascades
Cell biology
Up-Regulation
Endothelial stem cell
Adult Stem Cells
Medicine
Stem cell
Adult stem cell
Research Article
Signal Transduction
Adult
Biology
Signaling Pathways
Cell Line
03 medical and health sciences
Cancer stem cell
Genetics
Calcium-Mediated Signal Transduction
Animals
Humans
Calcium Signaling
030304 developmental biology
Myocardium
lcsh:R
Calcium-Binding Proteins
Computational Biology
Mesenchymal Stem Cells
Coculture Techniques
Rats
Calcium Signaling Cascade
Immunology
Trans-Activators
lcsh:Q
Stem Cell Lines
030217 neurology & neurosurgery
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 6, p e38454 (2012)
DOI: 10.17615/dyj3-fy47
Popis: The phenotype of somatic cells has recently been found to be reversible. Direct reprogramming of one cell type into another has been achieved with transduction and over expression of exogenous defined transcription factors emphasizing their role in specifying cell fate. To discover early and novel endogenous transcription factors that may have a role in adult-derived stem cell acquisition of a cardiomyocyte phenotype, mesenchymal stem cells from human and mouse bone marrow and rat liver were co-cultured with neonatal cardiomyocytes as an in vitro cardiogenic microenvironment. Cell-cell communications develop between the two cell types as early as 24 hrs in co-culture and are required for elaboration of a myocardial phenotype in the stem cells 8-16 days later. These intercellular communications are associated with novel Ca(2+) oscillations in the stem cells that are synchronous with the Ca(2+) transients in adjacent cardiomyocytes and are detected in the stem cells as early as 24-48 hrs in co-culture. Early and significant up-regulation of Ca(2+)-dependent effectors, CAMTA1 and RCAN1 ensues before a myocardial program is activated. CAMTA1 loss-of-function minimizes the activation of the cardiac gene program in the stem cells. While the expression of RCAN1 suggests involvement of the well-characterized calcineurin-NFAT pathway as a response to a Ca(2+) signal, the CAMTA1 up-regulated expression as a response to such a signal in the stem cells was unknown. Cell-cell communications between the stem cells and adjacent cardiomyocytes induce Ca(2+) signals that activate a myocardial gene program in the stem cells via a novel and early Ca(2+)-dependent intermediate, up-regulation of CAMTA1.
Databáze: OpenAIRE
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