Comparison of In Vitro Antimicrobial Susceptibility inFlavobacterium psychrophilumIsolated from Rainbow Trout Fry
| Autor: | Cavit Kum, Şükrü Kirkan, Murat Boyacioglu, Ferda Akar, Selim Sekkin |
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| Rok vydání: | 2008 |
| Předmět: |
Florfenicol
Colony Count Microbial Flavobacterium psychrophilum Microbial Sensitivity Tests Aquatic Science Biology Flavobacterium Sensitivity and Specificity Microbiology Agar dilution Fish Diseases chemistry.chemical_compound Minimum inhibitory concentration Flavobacteriaceae Infections Drug Resistance Bacterial Enrofloxacin medicine Animals Agar diffusion test Dose-Response Relationship Drug Antimicrobial Anti-Bacterial Agents Treatment Outcome chemistry Oncorhynchus mykiss Rainbow trout medicine.drug |
| Zdroj: | Journal of Aquatic Animal Health. 20:245-251 |
| ISSN: | 1548-8667 0899-7659 |
| Popis: | The aim of this study was to demonstrate the presence of Flavobacterium psychrophilum in the west Aegean region of Turkey and to evaluate the in vitro susceptibility of F. psychrophilum (isolated from the fry of rainbow trout Oncorhynchus mykiss) to seven antimicrobial agents, as determined by the disk diffusion and agar dilution methods. A total of 250 rainbow trout fry (weight = 2-5 g; total length = 3-6 cm) were examined, and 20 bacterial isolates were phenotypically identified. Antimicrobial agents included in this investigation were amoxicillin-clavulanic acid (AMC), erythromycin (E), enrofloxacin (ENR), florfenicol (FFC), gentamicin (CN), oxytetracycline (OT), and sulfamethoxazole-trimethoprim (SXT). Disk diffusion and agar dilution methods were performed according to published standards. Minimum inhibitory concentration (MIC) ranges were determined using the agar dilution method for F. psychrophilum isolates. Resistance of F. psychrophilum to CN (disk diffusion method: 70%; agar dilution method: 95%), E (65%; 100%), and SXT (75%; 100%) was high using both methods. Resistance to ENR (10%; 15%) and FFC (25%; 25%) was low with both methods; MIC90 (minimum concentration required to inhibit bacterial growth by 90%) was 4 microg/mL for ENR and 16 microg/mL for FFC. Ninety percent of the F. psychrophilum isolates were resistant to AMC based on the disk diffusion method, while only 15% of isolates showed resistance based on the agar dilution method. For OT, 20% of isolates were resistant based on disk diffusion, while 75% exhibited resistance based on agar dilution. The importance of susceptibility testing when facing an outbreak of F. psychrophilum at a fish farm is obvious; however, the discrepancies between testing methods for AMC and OT require further studies. |
| Databáze: | OpenAIRE |
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