Production of ginsenoside aglycons and Rb1 deglycosylation pathway profiling by HPLC and ESI-MS/MS using Sphingobacterium multivorum GIN723
| Autor: | Duck-Hee Kim, Eun-Mi Kim, Jun-Seong Park, Juhan Kim, Byung-Gee Kim, Joo-Hyun Seo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Glycosylation Sapogenins Chromatography Ginsenosides Molecular Structure Sphingobacterium multivorum Plant Extracts Chemistry Electrospray ionization General Medicine Applied Microbiology and Biotechnology High-performance liquid chromatography Ginseng chemistry.chemical_compound Enzyme Biochemistry Biotransformation Tandem Mass Spectrometry Ginsenoside Sphingobacterium Specific activity Chromatography High Pressure Liquid Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 97:8031-8039 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-013-4982-y |
| Popis: | Using enrichment culture, Sphingobacterium multivorum GIN723 (KCCM80060) was isolated as having activity for deglycosylation of compound K and ginsenoside F1 to produce ginsenoside aglycons such as S-protopanaxadiol (PPD(S)) and S-protopanaxatriol (PPT(S)). Through BLAST search, purified enzyme from S. multivorum GIN723 was revealed to be the outer membrane protein. The purified enzyme from S. multivorum GIN723 has unique specificity for the glucose moiety. However, it has activity with PPD and PPT group ginsenosides such as ginsenosides Rb1, Rb2, Rb3, Rc, F2, CK, Rh2, Re, and F1. From these results, it was predicted that the enzyme has activity on several ginsenosides. Therefore, the biotransformation pathway from Rb1, which is a major, highly glycosylated compound of ginseng, was analyzed using high-performance liquid chromatography and electrospray ionization mass spectrometry/mass spectrometry. The dominant biotransformation pathway from Rb1 to PPD(S) was determined to be Rb1 → Gp-XVII → Gp-LXXV → CK → PPD(S). S. multivorum GIN723 can be used as a whole cell biocatalyst because its activity as whole cells is nine times higher than its activity as cell extracts. The specific activity of whole cells is 2.89 nmol/mg/min in the production of PPD(S). On the other hand, the specific activity of cell extracts is 0.32 nmol/mg/min. The productivity of this enzyme in whole cell form is 500 mg/1 l of cultured cell. Its optimum reaction condition is 10 mM of calcium ions added to a phosphate buffer with a pH of 8.5. |
| Databáze: | OpenAIRE |
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