Hepatoprotective and antioxidant effects of the coffee diterpenes kahweol and cafestol on carbon tetrachloride-induced liver damage in mice
| Autor: | Hye Gwang Jeong, Jea Ho Choi, Kyungjin Lee |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment Cafestol Toxicology medicine.disease_cause Antioxidants Gene Expression Regulation Enzymologic Lipid peroxidation Mice chemistry.chemical_compound Superoxides medicine Animals Carbon Tetrachloride Kahweol Mice Inbred ICR Molecular Structure Liver Diseases Cytochrome P-450 CYP2E1 Free Radical Scavengers General Medicine Glutathione CYP2E1 Liver chemistry Biochemistry Carbon tetrachloride Lipid Peroxidation Chemical and Drug Induced Liver Injury Diterpenes Oxidative stress Food Science medicine.drug |
| Zdroj: | Food and Chemical Toxicology. 45:2118-2125 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2007.05.010 |
| Popis: | The hepatoprotective effects of kahweol and cafestol, coffee-specific diterpenes, on the carbon tetrachloride (CCl(4))-induced liver damage as well as the possible mechanisms involved in these protections were investigated. Pretreatment with kahweol and cafestol prior to the administration of CCl(4) significantly prevented the increase in the serum levels of hepatic enzyme markers (alanine aminotransferase and aspartate aminotransferase) and reduced oxidative stress, such as reduced glutathione content and lipid peroxidation, in the liver in a dose-dependent manner. The histopathological evaluation of the livers also revealed that kahweol and cafestol reduced the incidence of liver lesions induced by CCl(4). Treatment of the mice with kahweol and cafestol also resulted in a significant decrease in the cytochrome P450 2E1 (CYP2E1), the major isozyme involved in CCl(4) bioactivation, specific enzyme activities, such as p-nitrophenol and aniline hydroxylation. Kahweol and cafestol exhibited antioxidant effects on FeCl(2)-ascorbate induced lipid peroxidation in a mouse liver homogenate, and on superoxide radical scavenging activity. These results suggest that the protective effects of kahweol and cafestol against the CCl(4)-induced hepatotoxicity possibly involve mechanisms related to their ability to block the CYP2E1-mediated CCl(4) bioactivation and free radical scavenging effects. |
| Databáze: | OpenAIRE |
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