Characterization and cloning of glycogen phosphorylase 1 from Dictyostelium discoideum
| Autor: | Charles L. Rutherford, Joseph F. Sucic, Patricia V. Rogers, Shun Luo |
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| Rok vydání: | 1992 |
| Předmět: |
Phosphorylases
Immunoblotting Molecular Sequence Data Restriction Mapping Biophysics Polymerase Chain Reaction Biochemistry Dictyostelium discoideum Glycogen phosphorylase Structural Biology Sequence Homology Nucleic Acid Genetics Animals Dictyostelium Amino Acid Sequence Cloning Molecular Peptide sequence Gene Base Sequence biology Nucleic acid sequence Intron RNA Fungal DNA biology.organism_classification genomic DNA Codon usage bias |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1129:262-272 |
| ISSN: | 0167-4781 |
| Popis: | We have cloned cDNA and genomic DNA fragments from Dictyostelium discoiseum that represent the entire coding egion of glycogen phosphorylase 1 (gpl, α- d -glucosyltransferase, EC 2.4.1.1). Nucleic acid sequencing of the gp1 clones revealed a single 139 bp intron separating the two exons that encode the 853 amino acids of gp1. The gp1 sequences are similar to other genes and proteins described for Dictyostelium in terms of G + C composition of coding and noncoding regions, splice junctions, intron length, codon preference and termination signals. Genomic Southern blot hybridizations suggest that gp1 exists as a single or low copy number gene in Dictyostelium. Northern analyses demonstrate that gp1 is a developmentally regulated transcript. In alignments of the gp1 peptide sequence to glycogen phosphorylase sequences from other organisms, a high degree of amino acid conservation at many active and regulatory sites was found; however, critical residues in the AMP and purine binding sites were not conserved. |
| Databáze: | OpenAIRE |
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