Evaluation of Genotoxic and DNA Photo-Protective Activity of Bryothamnion triquetrum and Halimeda incrassata Seaweeds Extracts
| Autor: | Fabiana Fuentes-León, André Passaglia Schuch, Carlos Frederico Martins Menck, Marioly Vernhes Tamayo, Angel Sánchez-Lamar, Maribel González-Pumariega |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
plasmid DNA
UV radiation DNA protection 0301 basic medicine Aging EcoRI Pharmaceutical Science Dermatology 030226 pharmacology & pharmacy lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Plasmid Plasmid dna Halimeda incrassata Botany Chemical Engineering (miscellaneous) Food science chemistry.chemical_classification biology Restriction enzyme 030104 developmental biology Enzyme chemistry lcsh:QD1-999 biology.protein Surgery DNA |
| Zdroj: | Cosmetics, Vol 4, Iss 3, p 23 (2017) Cosmetics; Volume 4; Issue 3; Pages: 23 |
| ISSN: | 2079-9284 |
| Popis: | The ultraviolet (UV) component of sunlight is high on the earth surface, especially at low latitudes, raising the risk of skin diseases, including cancer. The use of natural compounds is a strategy to protect people against UV damage. Seaweeds are becoming increasingly influential in the food industry, and are also used in the pharmacy and cosmetic industries, due to several bioactive demonstrated properties. This work analyzed the genotoxic and photoprotective effects of the aqueous extracts of two seaweed species: Bryothamnion triquetrum and Halimeda incrassata. A cell-free plasmid DNA assay was employed, allowing detection of DNA breaks. The plasmids were exposed to increasing concentrations of aqueous extracts. DNA break was produced at concentrations of 2.0 and 4.0 mg/mL in both seaweed extracts and, consequently, a genotoxic effect is postulated. This effect arises with higher exposure times. Additionally, different combinations of plasmid DNA, restriction enzymes (Eco RI, Bam HI, and Pvu II) and extracts were assayed. The extracts did not produce an interference effect in the reconnaissance of the specific restriction target sequences of each enzyme. Photoprotective activity of the extracts was evaluated in UVC-irradiated plasmids. None of the extracts displayed DNA protective effects in this assay. |
| Databáze: | OpenAIRE |
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