A Sensitive HPTLC Method for the Estimation of Glibenclamide, Rosiglitazone Maleate and Metformin Hydrochloride from a Multicomponent Dosage Form
| Autor: | Konde Abbulu, Swati D. Bhende, Murali Balaram Varanasi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Documentation system
01 natural sciences Dosage form Analytical Chemistry Rosiglitazone Glibenclamide 03 medical and health sciences 0302 clinical medicine Limit of Detection Glyburide medicine 030212 general & internal medicine Routine analysis Chromatography High Pressure Liquid Rosiglitazone Maleate Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine Metformin Hydrochloride Metformin 0104 chemical sciences Stationary phase Calibration Chromatography Thin Layer Uv detection Tablets medicine.drug |
| Zdroj: | Journal of Chromatographic Science. 58:418-426 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/bmz124 |
| Popis: | A sensitive, rapid and cost-effective method based on HPTLC with UV detection was developed for the quantitation of Glibenclamide (GLIBEN), Rosiglitazone maleate (ROSI) and Metformin hydrochloride (MET) from a combined dosage form. Pre-coated RP-18 F254s aluminum sheets were used as the stationary phase. Methanol–tetrahydrofuran–water–glacial acetic acid (16: 3.6: 4: 0.4, v/v) used as the mobile phase, along with chamber saturation of 10 min offered an optimum migration (Rf = 0.54, 0.62 and 0.80 for GLIBEN, ROSI and MET, respectively). TLC Scanner 3 was used for densitometric evaluation of the chromatograms. DigiStore 2 Documentation System with winCATS software version 1.4.10 was used for the quantitation and photodocumentation. The LOD for GLIBEN, ROSI and MET was found to be 80 ng, 80 ng and 48 ng, respectively. Moreover, the LOQ was 200 ng, 200 ng and 120 ng for GLIBEN, ROSI and MET, respectively. The method was linear for GLIBEN (r = 0.9991), ROSI (r = 0.9993) and MET (r = 0.9988) within the tested range (200–1000, 200–1000 and 120–600 ng/band, respectively). The method was found to be precise and accurate for all the three drugs. The method was applied for the analysis of Triglucored tablets, and it proved to be a reliable quality control tool for the routine analysis of GLIBEN, ROSI and MET in a combined dosage form. |
| Databáze: | OpenAIRE |
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