Expression of the genes encoding CCAAT-enhancer binding protein isoforms in the mouse mammary gland during lactation and involution
| Autor: | Gareth E. Davies, Maria Grosse, Anthony Cryer, Dipak Purshottam Ramji, Georgios Sabatakos |
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| Rok vydání: | 1998 |
| Předmět: |
Gene isoform
medicine.medical_specialty Transcription Genetic Mammary gland Biology Polymerase Chain Reaction Biochemistry Mice Mammary Glands Animal Lactation Internal medicine Gene expression medicine Animals Involution (medicine) RNA Messenger Molecular Biology Transcription factor Mice Inbred BALB C Ccaat-enhancer-binding proteins Nuclear Proteins Cell Biology DNA-Binding Proteins AP-1 transcription factor Endocrinology medicine.anatomical_structure Gene Expression Regulation CCAAT-Enhancer-Binding Proteins Female Transcription Factors Research Article |
| Zdroj: | Biochemical Journal. 334:205-210 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the activation of gene expression in the mammary gland during lactation. We have therefore investigated the detailed expression profile of the C/EBP family during lactation and involution of the mouse mammary gland. The expression of C/EBPβ and C/EBPδ mRNA was low during lactation, increased dramatically at the beginning of involution and remained constant thereafter. In contrast, C/EBPα mRNA expression was relatively high during the early stages of lactation, declined to low levels during the late stages of lactation and at the start of involution, and increased again during involution. Electrophoretic mobility-shift assays showed a close correlation between the expression of the C/EBP genes and the functional C/EBP DNA-binding activity and, additionally, demonstrated the participation of heterodimers, formed from among the three proteins, in DNA–protein interactions. The DNA-binding activity of the activator protein 1 (AP1) family of transcription factors was also induced during involution. These results therefore point to potentially important regulatory roles for both the C/EBP and the AP1 family during lactation and involution of the mammary gland. |
| Databáze: | OpenAIRE |
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