Sequential analysis and staging of a diethylnitrosamine-induced hepatocellular carcinoma in male Wistar albino rat model
| Autor: | Ahmed M. Mansour, Bakheet E. M. Elsadek, Mohamad Abdel-Monem Bashandy, Abdallah Fathy, Samir A. E. Bashandy |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Pathology Carcinoma Hepatocellular Physiology Rat model medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Physiology (medical) Internal medicine Animals Medicine Diethylnitrosamine Rats Wistar Staging system Neoplasm Staging Pharmacology Hematology medicine.diagnostic_test business.industry Body Weight Liver Neoplasms Organ Size General Medicine medicine.disease Lipids digestive system diseases Rats Disease Models Animal Oxidative Stress 030104 developmental biology Liver Morphometric analysis 030220 oncology & carcinogenesis Hepatocellular carcinoma Liver function business Lipid profile Oxidative stress |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 95:1462-1472 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/cjpp-2017-0413 |
| Popis: | Hepatocellular carcinoma (HCC) is one of the most life-threatening cancers. The present study was designed to chronologically analyze the HCC chemically induced by diethylnitrosamine (DEN) in male Wistar rats during a 27-week period. DEN was given to rats in drinking water (100 mg/L) to induce HCC. In the present study, the DEN-administered groups recorded dramatic results in the tumor markers, oxidative stress, lipid profile, liver function, and hematological parameters at all intervals when compared with their corresponding values in the control groups. In addition, the morphometric analysis of livers of the DEN-administered groups (from 9 to 27 weeks) showed gradual enlargement and several grayish white nodules and foci on the peripheral surface of the liver as the features of HCC. In conclusion, the present sequential model chronologically analyzes all steps of hepatocarcinogenesis and presents a new staging system for classification of HCC that may be valuable for investigating the effects of anticarcinogenic compounds at varying stages of hepatocarcinogenesis in vivo. |
| Databáze: | OpenAIRE |
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