Development of novel application of 3,3′-diindolylmethane: sensitizing multidrug resistance human breast cancer cells to γ-irradiation
| Autor: | Jingang Zhang, Wenjing Wang, Maomin Lv, Yanlin Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Radiation-Sensitizing Agents 3 3'-Diindolylmethane Indoles Pharmaceutical Science Breast Neoplasms Biology Pharmacology Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Discovery medicine Anticarcinogenic Agents Humans MTT assay Propidium iodide Dose-Response Relationship Drug medicine.diagnostic_test General Medicine Cell cycle Drug Resistance Multiple Multiple drug resistance 030104 developmental biology Complementary and alternative medicine chemistry Drug Resistance Neoplasm Gamma Rays Apoptosis 030220 oncology & carcinogenesis Cancer cell MCF-7 Cells Cancer research Molecular Medicine Female |
| Zdroj: | Pharmaceutical Biology. 54:3164-3168 |
| ISSN: | 1744-5116 1388-0209 |
| Popis: | Context: Multidrug resistance (MDR) is known as a major obstacle to effective cancer therapy. The effects of irradiation on MDR in cancer cells had rarely been reported.Objective: The effect of 3,3′-diindolylmethane (DIM) sensitizing MDR human breast carcinoma to γ-irradiation was investigated.Materials and methods: MCF-7/ADR cells were exposed to different concentrations of DIM (0–30 μM) for 48 or 2 h before IR (γ-Co60, 10 Gy, room temperature) then cultured for 48 h. Cell survival was determined by MTT assay. Intracellular reactive oxygen spices (ROS) induced by DIM (20 and 30 μM, 2 h before irradiation) was measured by flow cytometry. Propidium iodide staining assay was used for cell cycle distribution studies; cell apoptosis was measured by flow cytometry and confocal microscopy.Results: DIM (20 and 30 μM, 2 h before irradiation) sensitized MCF-7/ADR cells to IR with survival rates decreased from 100% to 79% and 63%, respectively. DIM combined with γ-radiation demonstrated that the activity of... |
| Databáze: | OpenAIRE |
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