New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates
| Autor: | Susan Maleki, Mali Mærk, Helga Ertesvåg, Radka Hrudikova, Svein Valla |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Alginates 030106 microbiology Fructose 6-phosphate Bioengineering Pseudomonas fluorescens Dehydrogenase Biology Pentose phosphate pathway Glucosephosphate Dehydrogenase Polysaccharide 03 medical and health sciences chemistry.chemical_compound Industrial Microbiology Glucuronic Acid Stress Physiological Glycerol Molecular Biology chemistry.chemical_classification Hexuronic Acids Fructose General Medicine biology.organism_classification Immunohistochemistry Biosynthetic Pathways 030104 developmental biology Glucose chemistry Biochemistry Genes Bacterial Bacterial outer membrane Biotechnology |
| Zdroj: | New biotechnology. 37 |
| ISSN: | 1876-4347 |
| Popis: | Alginate denotes a family of linear polysaccharides with a wide range of industrial and pharmaceutical applications. Presently, all commercially available alginates are manufactured from brown algae. However, bacterial alginates have advantages with regard to compositional homogeneity and reproducibility. In order to be able to design bacterial strains that are better suited for industrial alginate production, defining limiting factors for alginate biosynthesis is of vital importance. Our group has been studying alginate biosynthesis in Pseudomonas fluorescens using several complementary approaches. Alginate is synthesised and transported out of the cell by a multiprotein complex spanning from the inner to the outer membrane. We have developed an immunogold labelling procedure in which the porin AlgE, as a part of this alginate factory, could be detected by transmission electron microscopy. No time-dependent correlation between the number of such factories on the cell surface and alginate production level was found in alginate-producing strains. Alginate biosynthesis competes with the central carbon metabolism for the key metabolite fructose 6-phosphate. In P. fluorescens, glucose, fructose and glycerol, are metabolised via the Entner-Doudoroff and pentose phosphate pathways. Mutational analysis revealed that disruption of the glucose 6-phosphate dehydrogenase gene zwf-1 resulted in increased alginate production when glycerol was used as carbon source. Furthermore, alginate-producing P. fluorescens strains cultivated on glucose experience acid stress due to the simultaneous production of alginate and gluconate. The combined results from our studies strongly indicate that the availability of fructose 6-phosphate and energy requires more attention in further research aimed at the development of an optimised alginate production process. |
| Databáze: | OpenAIRE |
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