Synthesis, Characterization and in vitro Anti-Tumoral Evaluation of Erlotinib-PCEC Nanoparticles
| Autor: | Davoud Asgari, Aylar Nakhlband, Hadi Valizadeh, Leila Barghi, Jaleh Barar |
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| Rok vydání: | 2015 |
| Předmět: |
Ethylene Oxide
Cancer Research Cell Survival Epidemiology Proton Magnetic Resonance Spectroscopy Nanoparticle In Vitro Techniques Ring-opening polymerization Erlotinib Hydrochloride Lactones Cell Line Tumor Spectroscopy Fourier Transform Infrared PEG ratio Copolymer medicine Humans MTT assay Protein Kinase Inhibitors Cell Proliferation Chemistry Public Health Environmental and Occupational Health ErbB Receptors Oncology Quinazolines Nanoparticles Particle size Erlotinib Drug Screening Assays Antitumor Nuclear chemistry medicine.drug |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 15:10281-10287 |
| ISSN: | 1513-7368 |
| Popis: | Background: Development of a nanosized polymeric delivery system for erlotinib was the main objective of this research. Materials and Methods: Poly caprolactone-polyethylene glycol-polycaprolactone (PCEC) copolymers with different compositions were synthesized via ring opening polymerization. Formation of triblock copolymers was confirmed by HNMR as well as FT-IR. Erlotinib loaded nanoparticles were prepared by means of synthesized copolymers with solvent displacement method. Results: Physicochemical properties of obtained polymeric nanoparticles were dependent on composition of used copolymers. Size of particles was decreased with decreasing the PCL/PEG molar ratio in used copolymers. Encapsulation efficiency of prepared formulations was declined by decreasing their particle size. Drug release behavior from the prepared nanoparticles exhibited a sustained pattern without a burst release. From the release profiles, it can be found that erlotinib release rate from polymeric nanoparticles is decreased by increase of CL/PEG molar ratio of prepared block copolymers. Based on MTT assay results, cell growth inhibition of erlotinib has a dose and time dependent pattern. After 72 hours of exposure, the 50% inhibitory concentration (IC50) of erlotinib hydrochloride was appeared to be 14.8 μM. Conclusions: From the obtained results, it can be concluded that the prepared PCEC nanoparticles in this study might have the potential to be considered as delivery system for erlotinib. |
| Databáze: | OpenAIRE |
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