Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon
| Autor: | Judith Sophie Weber, Sen Claudine Henriette Ngomtcho, Mbunkah Daniel Achukwi, Elisabeth Ngo Bum, Sørge Kelm, Thaddeus Terlumun Gbem |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Trypanosoma Veterinary medicine Tsetse Flies ITS1 030231 tropical medicine Prevalence Cattle Diseases Polymerase Chain Reaction Parasite Load Trypanosoma theileri lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Trypanosomiasis DNA Ribosomal Spacer parasitic diseases Northern Cameroon medicine Animals Mass Screening lcsh:RC109-216 Cameroon Trypanosoma grayi Bodonidae Mass screening Molecular Epidemiology biology Trypanosomosis Tsetse fly Research fungi Sequence Analysis DNA DNA Protozoan biology.organism_classification medicine.disease 030104 developmental biology Infectious Diseases Parasitology Cattle Nested polymerase chain reaction |
| Zdroj: | Parasites & Vectors, Vol 10, Iss 1, Pp 1-16 (2017) Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Background African trypanosomes are mainly transmitted through the bite of tsetse flies (Glossina spp.). The present study investigated the occurrence of pathogenic trypanosomes in tsetse flies and cattle in tsetse fly-infested areas of Northern Cameroon. Results Trypanosomes were identified using nested polymerase chain reaction (PCR) analysis of internal transcribed spacer 1 (ITS1) region, both by size estimation and sequencing of PCR products. Apparent density indices recorded in Gamba and Dodeo were 3.1 and 3.6 tsetse flies per trap and day, respectively. Trypanosoma prevalence infection rate for the tsetse fly gut (40%) and proboscis (19%) were recorded. Among the flies where trypanosomes were detected in the gut, 41.7% were positive for T. congolense and 14.6% for T. brucei ssp., whereas in the proboscis 36% harboured T. congolense and 62% contained T. vivax. T. grayi was highly prevalent in tsetse fly gut (58%). The most common mixed infections were the combination of T. congolense and T. grayi. Trypanosome prevalence rate in cattle blood was 6%. Among these, T. vivax represented 26%, T. congolense 35%, T. brucei ssp. 17% and T. theileri 17% of the infections. Surprisingly, in one case T. grayi was found in cattle. The mean packed cell volume (PCV) of cattle positive for trypanosomes was significantly lower (24.1 ± 5.6%; P |
| Databáze: | OpenAIRE |
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