The Mi-9 gene from Solanum arcanum conferring heat-stable resistance to root-knot nematodes is a homolog of Mi-1
| Autor: | Sophie Mantelin, Philip A. Roberts, Kishor Bhattarai, Oscar Martinez de Ilarduya, Isgouhi Kaloshian, Jetty S.S. Ammiraju, Barbara Jablonska |
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| Rok vydání: | 2006 |
| Předmět: |
Hot Temperature
Physiology Agrobacterium Nicotiana tabacum Solanum arcanum Plant Science Biology Solanum Chromosomes Plant Gene Expression Regulation Plant Genetics Homologous chromosome Animals Gene Silencing Tylenchoidea Gene Phylogeny Plant Diseases Plant Proteins Intron food and beverages Chromosome Mapping biology.organism_classification Solanaceae Research Article |
| Zdroj: | Plant physiology. 143(2) |
| ISSN: | 0032-0889 |
| Popis: | Resistance conferred by the Mi-1 gene from Solanum peruvianum is effective and widely used for limiting root-knot nematode (Meloidogyne spp.) yield loss in tomato (Solanum lycopersicum), but the resistance is ineffective at soil temperatures above 28°C. Previously, we mapped the heat-stable resistance gene Mi-9 in Solanum arcanum accession LA2157 to the short arm of chromosome 6, in a genetic interval as Mi-1 and the Cladosporium fulvum resistance gene Cf2. We developed a fine map of the Mi-9 region by resistance and marker screening of an F2 population and derived F3 families from resistant LA2157 × susceptible LA392. Mi-1 intron 1 flanking primers were designed to amplify intron 1 and fingerprint Mi-1 homologs. Using these primers, we identified seven Mi-1 homologs in the mapping parents. Cf-2 and Mi-1 homologs were mapped on chromosome 6 using a subset of the F2. Cf-2 homologs did not segregate with Mi-9 resistance, but three Mi-1 homologs (RH1, RH2, and RH4) from LA2157 and one (SH1) from LA392 colocalized to the Mi-9 region. Reverse transcriptase-polymerase chain reaction analysis indicated that six Mi-1 homologs are expressed in LA2157 roots. We targeted transcripts of Mi-1 homologs for degradation with tobacco (Nicotiana tabacum) rattle virus (TRV)-based virus-induced gene silencing using Agrobacterium infiltration with a TRV-Mi construct. In most LA2157 plants infiltrated with the TRV-Mi construct, Mi-9-meditated heat-stable root-knot nematode resistance was compromised at 32°C, indicating that the heat-stable resistance is mediated by a homolog of Mi-1. |
| Databáze: | OpenAIRE |
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