CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

Autor:	Jana Kerver, Selma Waaijers, Vincent Portegijs, Mike Boxem, Marcel Tijsterman, Bennie B. L. G. Lemmens, Sander van den Heuvel
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	double-strand break Molecular Sequence Data Mutagenesis (molecular biology technique) Biology Genome Genome engineering Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Notes Genetics CRISPR Animals Caenorhabditis elegans Cas9 Gene 030304 developmental biology 0303 health sciences Genome Helminth Base Sequence Methods Technology and Resources DNA Helminth biology.organism_classification Directed mutagenesis Gene Targeting Mutagenesis Site-Directed CRISPR-Cas Systems genome engineering 030217 neurology & neurosurgery
Zdroj:	Genetics Genetics, 195(3), 1187
Popis:	The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3087a8b9536584d884c6bdca2dabe1d3 https://hdl.handle.net/1887/101124 Zobrazit plný text záznamu