| Autor: |
Yong Guk Kang, Raymart Jay E. Canoy, Yongjun Jang, Ana Rita M. P. Santos, Inwoo Son, Beop-Min Kim, Yongdoo Park |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Biomed Opt Express |
| ISSN: |
2156-7085 |
| Popis: |
Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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