Modulation of Wnt signaling is essential for the differentiation of ciliated epithelial cells in human airways
| Autor: | Matthias Salathe, Juliette Sailland, Nathalie Baumlin, Andreas Schmid, Lisa Novak, Nevis Fregien |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
ciliated cells Indoles Dickkopf Biophysics Respiratory Mucosa Biology Cell Fate Determination Biochemistry Maleimides Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Structural Biology GSK-3 Research Letter Genetics medicine Humans Cilia Wnt Signaling Pathway Molecular Biology Cells Cultured Glycogen Synthase Kinase 3 beta Air liquid interface Wnt signaling pathway airway epithelium Cell Differentiation Epithelial Cells differentiation Cell Biology medicine.disease Wnt signaling Research Letters Epithelium Squamous metaplasia Cell biology 030104 developmental biology medicine.anatomical_structure DKK1 030220 oncology & carcinogenesis Intercellular Signaling Peptides and Proteins Respiratory epithelium |
| Zdroj: | Febs Letters |
| ISSN: | 0014-5793 |
| DOI: | 10.1002/1873-3468.12851 |
| Popis: | Wnt signaling is essential for the differentiation of airway epithelial cells during development. Here, we examined the role of Wnt signaling during redifferentiation of ciliated airway epithelial cells in vitro at the air liquid interface as a model of airway epithelial repair. Phases of proliferation and differentiation were defined. Markers of squamous metaplasia and epithelial ciliation were followed while enhancing β-catenin signaling by blocking glycogen synthase kinase 3β with SB216763 and shRNA as well as inhibiting canonical WNT signaling with apical application of Dickkopf 1 (Dkk1). Our findings indicate that enhanced β-catenin signaling decreases the number of ciliated cells and causes squamous changes in the epithelium, whereas treatment with DDk1 leads to an increased number of ciliated cells. |
| Databáze: | OpenAIRE |
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