Molecular cloning and functional characterization of a soybean GmGMP1 gene reveals its involvement in ascorbic acid biosynthesis and multiple abiotic stress tolerance in transgenic plants
| Autor: | Chenchen Xue, Jinming Zhao, Can Wang, Dong Xue, Han Xing, Na Guo, Jin-feng Hou, Jin-yan Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agriculture (General) superoxide anion radical Plant Science Genetically modified crops L-ascorbic acid 01 natural sciences Biochemistry S1-972 03 medical and health sciences chemistry.chemical_compound Food Animals Biosynthesis Arabidopsis Gene salt stress Ecology biology GDP-mannose pyrophosphorylase Abiotic stress Superoxide chlorophyll contents food and beverages Ascorbic acid biology.organism_classification 030104 developmental biology chemistry Glycine Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 17, Iss 3, Pp 539-553 (2018) |
| ISSN: | 2095-3119 |
| Popis: | L-Ascorbic acid (AsA) plays an important role in plants and animals. In plants, GDP-D-mannose pyrophosphorylase (GMP) is essential in the AsA biosynthetic pathway. However, little is known about the genes encoding GMP in soybean and here we report genetic and functional analysis of the GmGMP1 (Glycine max GDP-D-mannose pyrophosphorylase 1) gene in this species. GmGMP1 encoded a GDP-mannose pyrophosphorylase and exhibited higher transcript levels in the leaf than in the root, stem, flower, and seed. Transcript of this gene was ubiquitous in the vegetative and reproductive organs, and was induced by abiotic stress and light. Increasing expression of GmGMP1 in Arabidopsis and soybean through an overexpressing approach caused pronounced enhancement of AsA content, and was implicated in lowering the superoxide anion radical content and lipid peroxidation levels in Arabidopsis, and conferring tolerance to osmotic and high salt stresses during seed germination. The present study represents the first systematic determination of soybean genes encoding GDP-mannose pyrophosphorylase and provides useful evidence for the functional involvement of GmGMP1 in control of AsA content and conferring tolerance to osmotic and salt stress. |
| Databáze: | OpenAIRE |
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