Growth hormone gene expression in canine normal growth plates and spontaneous osteosarcoma
| Autor: | Elpetra P. M. Timmermans-Sprang, Jan A. Mol, Jolle Kirpensteijn, Gerard R. Rutteman, Irma S Lantinga-van Leeuwen, Evert van Garderen |
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| Rok vydání: | 2002 |
| Předmět: |
musculoskeletal diseases
Male medicine.medical_specialty medicine.medical_treatment Gene Expression Bone Neoplasms Biology Biochemistry Canine Osteosarcoma Endocrinology Dogs Internal medicine Gene expression medicine Animals Dog Diseases Growth Plate RNA Messenger Insulin-Like Growth Factor I Molecular Biology Messenger RNA Osteosarcoma Bone Development Osteoid Growth factor Osteoblast medicine.disease medicine.anatomical_structure Growth Hormone Immunohistochemistry Female |
| Zdroj: | Molecular and cellular endocrinology. 197(1-2) |
| ISSN: | 0303-7207 |
| Popis: | The indirect growth-promoting action of pituitary-derived growth hormone (GH) on skeletal growth is thought to be mediated by systemically released insulin-like growth factor-I (IGF-I) and by locally produced IGF-I. The aim of the present study was to document whether GH is expressed locally in canine bone and spontaneous osteosarcoma. Using RT-PCR the expression of GH mRNA was demonstrated in the metaphyseal, but not in the majority of epiphyseal ends of the canine growth plate. GH mRNA was also present in 25% of the canine osteosarcoma specimens. The expression of GH mRNA in predominantly active osteoid forming areas was associated with the presence of immunoreactive GH in osteoblasts, as shown by immunohistochemistry. It is concluded that locally produced GH is involved in osteoid formation and may play a role in the growth of neoplastic bone lesions in the dog. |
| Databáze: | OpenAIRE |
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