Identification of a cytoskeleton-associated glycoprotein from isolated microvilli of a mammary ascites tumor
| Autor: | Julie R. Craik, Kermit L. Carraway, Coralie A. Carothers Carraway, Goeh Jung, Robert W. Rubin |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Neuraminidase
Microfilament Cell Line Silver stain chemistry.chemical_compound Glucosamine Myosin Animals Lactoperoxidase Cytoskeleton Glycoproteins chemistry.chemical_classification Deoxyribonucleases Microvilli biology Cell Membrane Ascites Mammary Neoplasms Experimental Cell Biology Molecular biology Rats Isoelectric point chemistry Biochemistry biology.protein Electrophoresis Polyacrylamide Gel Isoelectric Focusing Glycoprotein |
| Zdroj: | Experimental Cell Research. 143:303-308 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(83)90055-1 |
| Popis: | Microvilli isolated from MAT-C1 13762 ascites tumor cells were extracted with Triton X-100 in phosphate-buffered saline (PBS) to yield cytoskeletal residues. Analysis of the residues by two-dimensional isoelectric focusing-dodecyl sulfate electrophoresis and silver staining suggested that one of the major components is a glycoprotein (CAG). Neuraminidase treatments and glucosamine labeling demonstrated that CAG is a glycoprotein, and lactoperoxidase iodination showed its presence at the microvillar surface. DNase treatments and myosin affinity analysis suggested an association between CAG and the microvillar microfilaments. Thus, CAG has the properties expected of a transmembrane-linking molecule connecting the cell surface to the cytoskeleton. |
| Databáze: | OpenAIRE |
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