Expression patterns and functional evaluation of RGMa during the early phase of peripheral nerve regeneration using the mouse median nerve model

Autor:	Hans-Eberhard Schaller, Peter Rosenberger, Martin Schäufele, Patrick Jaminet, Giulia Ronchi, Michele Fornaro, David Köhler, Alice Mager, Stefano Geuna
Rok vydání:	2019
Předmět:	030506 rehabilitation Pathology medicine.medical_specialty Transgene Mice Transgenic Nerve Tissue Proteins RGMa Motor Activity GPI-Linked Proteins grasping test Median nerve model peripheral nervous system transgenic 03 medical and health sciences Mice 0302 clinical medicine Developmental Neuroscience Western blot Peripheral nerve Peripheral Nerve Injuries medicine Animals RNA Messenger Messenger RNA medicine.diagnostic_test Behavior Animal business.industry Regeneration (biology) Repulsive guidance molecule A Median nerve Median Nerve Nerve Regeneration Disease Models Animal medicine.anatomical_structure Neurology Peripheral nervous system Neurology (clinical) 0305 other medical science business 030217 neurology & neurosurgery
Zdroj:	Restorative neurology and neuroscience. 37(3)
ISSN:	1878-3627
Popis:	BACKGROUND In this study, we evaluate the role of RGMa (Repulsive Guidance Molecule a) during peripheral nerve regeneration using the mouse median nerve model. METHODS By real-time PCR and Western Blot analysis, we examined expression changes of RGMa mRNA and RGMa protein in neural tissue after transection and microsurgical repair of the mouse median nerve distal to the transection site. We evaluated histomorphometrical changes in neural tissue distal to the injury site and functional recovery of the grasping force after median nerve transection and repair in wild-type mice and RGMa+/- heterozygous mice. RESULTS RT-PCR revealed a 1,8 fold increase of RGMa mRNA two weeks and a 4,4 fold increase of RGMa mRNA 3 weeks after nerve transection and repair in the nerve segment distal to the injury site. In Western blot analysis, we could show a high increase of RGMa in the nerve segment distal to the injury site at day 14. Histomorphometrical analysis showed significant differences between wild-type animals and heterozygous animals. The absolute number of myelinated fibres was significantly higher in operated heterozygous RGMa+/- animals compared to operated wildtye animals. Using the functional grasping test, we could demonstrate that peripheral nerve regeneration is significantly diminished in heterozygous RGMa+/- mice. CONCLUSIONS Employing the mouse median nerve model in transgenic animals, we demonstrate that RGMa plays an important role during peripheral nerve regeneration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30807c4e1f3cbb342ffb3b5147bd2711 https://pubmed.ncbi.nlm.nih.gov/31177252 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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