Bioengineering of living renal membranes consisting of hierarchical, bioactive supramolecular meshes and human tubular cells
| Autor: | Patricia Y. W. Dankers, Anton W. Bosman, Martin C. Harmsen, W. Meijer, Wilco P. J. Appel, Jasper M. Boomker, Eva Wisse, Ali Huizinga-van der Vlag, Frank M.M. Smedts, Marja J. A. van Luyn |
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| Přispěvatelé: | Macro-Organic Chemistry, Macromolecular and Organic Chemistry, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Vascular Ageing Programme (VAP) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Materials science
BASEMENT-MEMBRANES LAMININ RECEPTOR Biophysics Supramolecular chemistry FIBRONECTIN Bioengineering Nanotechnology ECM (extracellular matrix) Kidney Bioactivity Basement Membrane Extracellular matrix CULTURE Tissue engineering BIOARTIFICIAL KIDNEY medicine Humans BIOMATERIALS Cells Cultured Basement membrane chemistry.chemical_classification MESENCHYMAL TRANSITION Tissue Engineering biology Membrane THERMOPLASTIC ELASTOMERS Epithelial Cells IN-VITRO Self assembly Epithelium Extracellular Matrix Fibronectin Supramolecular polymers Epithelial cell Kidney Tubules medicine.anatomical_structure DIFFERENTIATION chemistry Mechanics of Materials Ceramics and Composites biology.protein |
| Zdroj: | Biomaterials, 32(3), 723-733. Elsevier Biomaterials, 32(3), 723-733. ELSEVIER SCI LTD |
| ISSN: | 1878-5905 0142-9612 |
| Popis: | Maintenance of polarisation of epithelial cells and preservation of their specialized phenotype are great challenges for bioengineering of epithelial tissues Mimicking the basement membrane and underlying extracellular matrix (ECM) with respect to its hierarchical fiber-like morphology and display of bioactive signals is prerequisite for optimal epithelial cell function in vitro We report here on a bottom-up approach based on hydrogen-bonded supramolecular polymers and ECM-peptides to make an electro-spun bioactive supramolecular mesh which can be applied as synthetic basement membrane The supramolecular polymers used self-assembled into nano-meter scale fibers while at micro-meter scale fibers were formed by electro-spinning We introduced bioactivity into these nano-fibers by intercalation of different ECM-peptides designed for stable binding Living kidney membranes were shown to be bioengineered through culture of primary human renal tubular epithelial cells on these bioactive meshes Even after a long-term culturing period of 19 days we found that the cells on bioactive membranes formed tight monolayers while cells on non-active membranes lost their monolayer integrity Furthermore the bioactive membranes helped to support and maintain renal epithelial phenotype and function Thus incorporation of ECM-peptides Into electro-spun meshes via a hierarchical supramolecular method is a promising approach to engineer bioactive synthetic membranes with an unprecedented structure This approach may in future be applied to produce living bioactive membranes for a I:no-artificial kidney (C) 2010 Elsevier Ltd All rights reserved |
| Databáze: | OpenAIRE |
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