Tachykinins activate nonselective cation currents in canine colonic myocytes
| Autor: | C. W. Shuttleworth, Hye Kyung Lee, Kenton M. Sanders |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Patch-Clamp Techniques Physiology Colon Neurokinin A Neuropeptide Substance P Peptide hormone Membrane Potentials chemistry.chemical_compound Dogs Internal medicine Cations medicine Carnivora Myocyte Animals Ion transporter Electric Conductivity Muscle Smooth Cell Biology In vitro Acetylcholine Endocrinology chemistry Biophysics Female Calcium Channels |
| Zdroj: | The American journal of physiology. 269(6 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | The mechanism of tachykinin-induced excitation was studied in isolated colonic muscle cells and intact muscle strips. In whole cell voltage-clamp studies performed at 33 degrees C, neurokinin A (NKA) and substance P (SP) reduced L-type Ca2+ current. NKA and SP activated a cationic current that reversed near 0 mV. This current (INKA or ISP, respectively) had properties similar to the acetylcholine (ACh)-activated nonselective cation conductance (IACh), activated by muscarinic stimulation in other gastrointestinal smooth muscle cells. INKA and ISP were decreased when external Na+ was reduced. In contrast to IACh, INKA and ISP were not facilitated by increases in internal Ca2+, but little or no current was activated by these peptides when extracellular Ca2+ was low. INKA (10(-7) M) and ISP (10(-5) M) were blocked by Cd2+ (5 x 10(-4) M), quinine (10(-3) M), and the tachykinin-receptor antagonist [D-Pro2,D-Trp7,9]SP (10(-5) M). Current clamp recordings and intracellular recordings of intact tissues showed that NKA and SP depolarized the cell membrane, which is consistent with the activation of a nonselective cation conductance. These data suggest that a primary mechanism of the tachykinins is to activate a nonselective cation conductance that leads to depolarization. The increase in Ca2+ entry due to tachykinin stimulation appears to be secondary to the activation of the nonselective cation conductance. |
| Databáze: | OpenAIRE |
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