T-Cell Receptor Identification of an Oligodendrocyte-Specific Autoreactive Cytotoxic T-Cell Clone Without Self Restriction
| Autor: | R. Lebar, S. Amzazi, M. A. Bach, Patrice N. Marche, V. Jewtoukoff |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Proteolipid protein 1
Receptors Antigen T-Cell alpha-beta Molecular Sequence Data Immunology Clone (cell biology) Autoimmunity Mice Inbred Strains Polymerase Chain Reaction Mice immune system diseases Sequence Homology Nucleic Acid medicine Animals Cytotoxic T cell Amino Acid Sequence Cloning Molecular Base Sequence biology T-cell receptor DNA General Medicine MHC restriction Flow Cytometry Molecular biology Oligodendrocyte Rats Myelin basic protein Oligodendroglia medicine.anatomical_structure Rats Inbred Lew biology.protein Alpha chain T-Lymphocytes Cytotoxic |
| Zdroj: | Scandinavian Journal of Immunology. 36:893-898 |
| ISSN: | 1365-3083 0300-9475 |
| Popis: | In addition to myelin basic protein (MBP) and proteolipid protein (PLP), oligodendrocyte (Od) membrane autoantigens, such as the glycoprotein M2/MOG, could participate in the pathogenesis of autoimmune demyelinating diseases of the central nervous system (CNS), such as experimental allergic encephalomyelitis (EAE) or multiple sclerosis (MS). We have described an Od-specific autoreactive and cytotoxic T-cell clone, named C2, which recognized M2/MOG without conventional MHC restriction. In order to analyse the Od/C2 interaction, we determined the alpha/beta T-cell receptor (TCR) variable region usages and structures of C2. Monoclonal antibody stainings of C2 and nucleotide sequences show that the alpha chain is composed of a V alpha 5 and a J alpha identical to J alpha 18BBM142 gene segments, and that the TCR beta chain is composed of V beta 17a, D beta 2.1 and J beta 2.2 gene segments indicating that C2 used a conventional alpha/beta TCR for M2/MOG recognition. |
| Databáze: | OpenAIRE |
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