Production, purification and characterization of antibodies to 1,25-dihydroxyvitamin D raised in chicken egg yolk
| Autor: | M.P. Devos, K.A. Van Brussel, J.A. Kint, R M Bauwens, A.P. De Leenheer |
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| Rok vydání: | 1987 |
| Předmět: |
Vitamin
food.ingredient Immunoglobulin gamma-Chains Clinical Biochemistry Radioimmunoassay Immunoelectrophoresis Biology Biochemistry High-performance liquid chromatography chemistry.chemical_compound food Calcitriol Antibody Specificity Yolk medicine Vitamin D and neurology Animals Bovine serum albumin Chromatography medicine.diagnostic_test Immune Sera Biochemistry (medical) General Medicine Egg Yolk chemistry biology.protein lipids (amino acids peptides and proteins) Antibody Chickens |
| Zdroj: | Clinica Chimica Acta. 170:37-44 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(87)90381-0 |
| Popis: | For this sensitive RIA for 1 alpha,25-dihydroxyvitamin D, we used antibodies to 1 alpha,25-dihydroxycholecalciferol-3-hemisuccinate conjugated to bovine serum albumin, raised in eggs by immunization of chickens. We describe an efficient method for purification of IgG from egg yolk. We characterized these antibodies with immunoelectrophoresis and by radioimmunoassay. These antibodies show a high affinity for 1 alpha,25-dihydroxyvitamin D3 but cross react with other vitamin D metabolites as well. Extraction and liquid chromatography are necessary to isolate the 1 alpha,25-dihydroxyvitamin D from human serum or plasma before determination by RIA. The sensitivity of the assay is estimated at 5 pg/tube. |
| Databáze: | OpenAIRE |
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