Fibroblast Growth Factor-1 Induces Phosphofructokinase, Fatty Acid Synthase and Ca2+-ATPase mRNA Expression in NIH 3T3 Cells
| Autor: | Patrick J. Donohue, Debbie K.W. Hsu, G.F. Alberts, Jeffrey A. Winkles |
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| Rok vydání: | 1993 |
| Předmět: |
Phosphofructokinase-1
Molecular Sequence Data Biophysics Gene Expression Calcium-Transporting ATPases Fibroblast growth factor Polymerase Chain Reaction Biochemistry 3T3 cells Mice Sequence Homology Nucleic Acid Gene expression medicine Animals Amino Acid Sequence RNA Messenger Fibroblast Molecular Biology DNA Primers Base Sequence biology Cell growth 3T3 Cells Cell Biology Oligonucleotides Antisense Molecular biology Fatty acid synthase medicine.anatomical_structure Enzyme Induction biology.protein Fibroblast Growth Factor 1 Fatty Acid Synthases Differential display technique Phosphofructokinase |
| Zdroj: | Biochemical and Biophysical Research Communications. 197:1483-1491 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.2644 |
| Popis: | Polypeptide growth factors act in part by inducing the expression of specific proteins that perform functions critical to cell cycle progression. We have used a differential display technique to identify genes that are expressed at higher levels following fibroblast growth factor (FGF)-1 (acidic FGF) stimulation of quiescent murine NIH 3T3 fibroblasts. Three such genes--liver (B-type) phosphofructokinase (PFK), fatty acid synthase (FAS) and sarco(endo)plasmic reticulum Ca(2+)-ATPase type 2 (SERCA2)--are described in this report. The level of FAS and SERCA2 mRNA expression is increased rapidly after FGF-1 addition; in contrast, PFK mRNA is induced with kinetics more typical of delayed-early genes. These results indicate that enhanced expression of the PFK, FAS and SERCA2 proteins may be important for FGF-1-stimulated cell proliferation. |
| Databáze: | OpenAIRE |
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