Immunophenotypic differences between neoplastic and non-neoplastic androgen-producing cells containing and lacking Reinke crystals
| Autor: | Scott R Gilles, Wendy Larson, Susan Dachel, Carlos Manivel, Hector Mesa, Paari Murugan, Milton W. Datta |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Pathology Adrenal Rest Tumor Adolescent medicine.drug_class macromolecular substances Biology Immunophenotyping Pathology and Forensic Medicine Young Adult 03 medical and health sciences 0302 clinical medicine Testicular Neoplasms Internal medicine Biomarkers Tumor medicine Humans Sex Cord-Gonadal Stromal Tumors Child Molecular Biology Aged Ovarian Neoplasms Leydig cell Leydig Cells Reinke crystals Cell Biology General Medicine Middle Aged medicine.disease Androgen Androgen receptor 030104 developmental biology Endocrinology medicine.anatomical_structure Receptors Androgen Calbindin 2 030220 oncology & carcinogenesis Adrenogenital syndrome Androgens Female Androgen insensitivity syndrome Calretinin |
| Zdroj: | Virchows Archiv. 469:679-686 |
| ISSN: | 1432-2307 0945-6317 |
| DOI: | 10.1007/s00428-016-2028-4 |
| Popis: | We performed a detailed morphologic, immunophenotypic, and endocrine characterization of neoplastic and non-neoplastic lesions of androgen-producing cells known to harbor or lack Reinke crystals (RCs) with an aim to provide further insight into the nature of these cells and crystals. Study cases were selected from the files of participating hospitals and subclassified according to current classifications: 20 with Leydig cell tumors (LCTs), 2 with testicular adrenal rest tumors (TARTs), 2 with testicular tumors of adrenogenital syndrome (TTAGS), and 2 with androgen insensitivity syndrome (AIS). An extensive immunophenotypic panel including markers used in sex cord-stromal cell tumors, androgen hormones, enzymes, and receptors was applied to the cases and 10 non-tumoral adrenal glands. Non-tumoral tissues were scored separately. RCs were present in 90 % of LCT cases and all cases with normal Leydig cells; RCs stained specifically with calretinin and 3β-hydroxysteroid dehydrogenase (3BHSD) and were present only in cells with high concomitant expression of both proteins, a phenotype unique to Leydig cells and LCTs. Leydig cells from AIS cases lack RCs due to decreased expression of 3BHSD. Calretinin is decreased in testicular adrenal-like tumors and absent in normal adrenocortical cells, which explain why they lack RCs. Calretinin expression in androgen-producing cells is independent from androgen receptors and androgen synthesis. RCs represent for the most part, if not exclusively, crystallized forms of a 3BHSD/calretinin complex. Androgen-producing cells containing and lacking RCs differ mainly in the level of expression of these proteins and androgen receptors. |
| Databáze: | OpenAIRE |
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