The protective role of DJ-1 in ultraviolet-induced damage of human skin: DJ-1 levels in the stratum corneum as an indicator of antioxidative defense
| Autor: | Noriko Noguchi, Shioji Ishiwatari, Maho Nakagawa, Shoko Matsukuma, Yoshiro Saito, Chie Yasuda, Takahashi Minako |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Keratinocytes Male Small interfering RNA Adolescent Ultraviolet Rays Protein Deglycase DJ-1 Skin Pigmentation Human skin Dermatology Biology medicine.disease_cause Antioxidants Cell Line Young Adult chemistry.chemical_compound In vivo Lactate dehydrogenase Stratum corneum medicine Humans RNA Small Interfering Oncogene Proteins chemistry.chemical_classification Reactive oxygen species L-Lactate Dehydrogenase integumentary system Intracellular Signaling Peptides and Proteins Hydrogen Peroxide General Medicine Middle Aged Molecular biology Cytoprotection Oxidative Stress medicine.anatomical_structure Biochemistry chemistry Cytokines Female RNA Interference Epidermis Biomarkers Oxidative stress |
| Zdroj: | Archives of Dermatological Research. 307:925-935 |
| ISSN: | 1432-069X 0340-3696 |
| Popis: | DJ-1 is a multifunctional protein associated with Parkinson's disease and plays a significant role in protecting nerve cells from oxidative stress. DJ-1 is expressed in the skin, although its function there is unknown. In this study, we investigated DJ-1 function in keratinocytes. DJ-1 was induced by H2O2 exposure and UV irradiation in keratinocytes. DJ-1 knockdown with small interfering RNA (siRNA) increased reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release after UVB irradiation, suggesting that DJ-1 reduces ROS and might protect skin cells from UV damage in vitro. To investigate the in vivo role of DJ-1 in the skin, we determined DJ-1 levels in human stratum corneum samples obtained by the tape-stripping method. DJ-1 levels in the stratum corneum (scDJ-1) correlated with total antioxidant capacity. We also examined the effect of scDJ-1 on changes in skin after UVB irradiation. DJ-1 was elevated in SC from the upper arm 1 to 2 weeks after UVB irradiation. One day after UVB irradiation, L* (brightness) and a* (redness) values, indicators of skin color, were altered regardless of scDJ-1 expression. However, these values recovered more quickly in subjects with high scDJ-1 expression than in those with low scDJ-1 expression. These data suggest that DJ-1 in skin plays a significant role in protection against UV radiation and oxidative stress, and that DJ-1 levels in the SC might be an indicator of antioxidative defense against UV-induced damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink