Comparative transcriptomic analysis of dermal wound healing reveals de novo skeletal muscle regeneration in Acomys cahirinus

Autor: Ruth Davenport, W. Brad Barbazuk, Aaron Gabriel W. Sandoval, J. Lucas Boatwright, Jason O. Brant, Malcolm Maden
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Physiology
Gene Expression
Muscle Development
Biochemistry
Transcriptome
White Blood Cells
Mice
0302 clinical medicine
Contractile Proteins
Animal Cells
Myosin
Morphogenesis
Medicine and Health Sciences
Musculoskeletal System
Skin
Multidisciplinary
biology
integumentary system
Muscles
Genomics
Panniculus carnosus
Cell biology
medicine.anatomical_structure
Medicine
Anatomy
Cellular Types
Transcriptome Analysis
Muscle Regeneration
Research Article
Science
Immune Cells
Immunology
Motor Proteins
Actin Motors
Myosins
03 medical and health sciences
Gene Types
Molecular Motors
Tissue Repair
medicine
Genetics
Regeneration
Animals
Muscle
Skeletal
Wound Healing
Blood Cells
Regeneration (biology)
Macrophages
Skeletal muscle
Biology and Life Sciences
Computational Biology
Proteins
Cell Biology
biology.organism_classification
Genome Analysis
Wnt Proteins
Cytoskeletal Proteins
030104 developmental biology
Spiny mouse
Skeletal Muscles
Myogenic regulatory factors
Regulator Genes
Murinae
Wound healing
Physiological Processes
Organism Development
030217 neurology & neurosurgery
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 5, p e0216228 (2019)
ISSN: 1932-6203
Popis: The African spiny mouse, Acomys spp., is capable of scar-free dermal wound healing. Here, we have performed a comprehensive analysis of gene expression throughout wound healing following full-thickness excisional dermal wounds in both Acomys cahirinus and Mus musculus. Additionally, we provide an annotated, de novo transcriptome assembly of A. cahirinus skin and skin wounds. Using a novel computational comparative RNA-Seq approach along with pathway and co-expression analyses, we identify enrichment of regeneration associated genes as well as upregulation of genes directly related to muscle development or function. Our RT-qPCR data reveals induction of the myogenic regulatory factors, as well as upregulation of embryonic myosin, starting between days 14 and 18 post-wounding in A. cahirinus. In contrast, the myogenic regulatory factors remain downregulated, embryonic myosin is only modestly upregulated, and no new muscle fibers of the panniculus carnosus are generated in M. musculus wounds. Additionally, we show that Col6a1, a key component of the satellite cell niche, is upregulated in A. cahirinus compared to M. musculus. Our data also demonstrate that the macrophage profile and inflammatory response is different between species, with A. cahirinus expressing significantly higher levels of Il10. We also demonstrate differential expression of the upstream regulators Wnt7a, Wnt2 and Wnt6 during wound healing. Our analyses demonstrate that A. cahirinus is capable of de novo skeletal muscle regeneration of the panniculus carnosus following removal of the extracellular matrix. We believe this study represents the first detailed analysis of de novo skeletal muscle regeneration observed in an adult mammal.
Databáze: OpenAIRE
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